Background:Dipeptidyl peptidase-9 (DPP9) is a serine exopeptidase that cleaves X-prolinedipeptides from the N-terminus of polypeptides. DPP9 exhibits similar activity tothat of DPP4 but unlike DPP4, DPP9 is not membrane bound. DPP9 activity has beenlinked to several diseases, including type 2 diabetes, obesity and cancer. Description:The FluorogenicDPP9 Assay Kit is designed to measure DPP9 activity using purified DPP9 for screeningand profiling applications. Thekey to the Fluorogenic DPP9 Assay Kit is the fluorogenic substrate. Using this kit,only one simple step on a microtiter plate is required for DPP9 reactions. The fluorometricsubstrate is incubated with a sample containing DPP9 enzyme to produce afluorophore that can then be measured using a fluorescence reader. Synonym(s): Dipeptidylpeptidase 9, DPPIX Supplied As: Supplied in a convenient 96-well format, with purified DPP9 enzyme, DPP substrate, and DPP assay buffer for 100 enzyme reactions. Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt Format: Instructions for use: See assay kit data sheet for detailed protocol. Storage / Stability:

At least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s): Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. Reference(s):

1. Bjelke,J.R. et al., Biochem. J. 396 (2), 391- 399 (2006). 2. Yu, D.M. et al., FEBS J. 273 (11), 2447-2460 (2006).

Application Reference(s):1.Screening of a natural compound library identifies emodin, a natural compound from Rheum palmatum Linn that inhibits DPP4(2017)

Notes: The AMC standard is included so the researcher can quatitatively determine thespecific activity of the enzyme using the AMC standard as a measure of how muchAMC substrate was cleaved to release free AMC. Warning(s): Avoid freeze/thaw cycles Scientific Category: Protease Product Type: Assay Kit Data shown is lot-specific. Contact us for specific information on other lots.

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