Species: Human Host Species: Sf9 insect cells MW: 42.4 kDa Genbank #: NM_018486 Tag(s): C-terminal His-tag a.a: full length Description:Human HDAC8 , GenBank Accession No. NM_018486, full length with C-terminal His-tag, MW= 46.4 kDa, expressed in a baculovirus expression system. UniProt Q9BY41 Synonym(s): histone deacetylase 8, HDAC-8 Specific Activity: ≥300 pmol/min/µg. Unit Definition: One U =1 pmol of acetyl group removed/min/µg of enzyme Assay Conditions: 25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl₂, and 0.1 mg/ml BSA, 20 µM BPS HDAC class 2a substrate (Catalog #50040), and HDAC8 (3.1 - 200 ng). Incubation condition: 30 min at 37°C, followed byHDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460. Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mMimidazole, and 10% glycerol. Format: Aqueous buffer solution Storage / Stability:

>6 months at -80°C.

Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Reference(s):

1. Lee, H. et al. Mol. Cell. Biol. 26 (14), 5259-5269 (2006).2. Gantt,S.L. et al., Biochemistry 45 (19), 6170-6178 (2006).

Application References: 1. An azumamide C analogue without the zinc-binding functionality (2017)2.The structural requirements of histone deacetylase inhibitors: C4-modified SAHA analogs display dual HDAC6/HDAC8 selectivity (2017)3.Impact of binding mechanism on selective inhibition of histone deacetylase isoforms (2017)4.Design, synthesis and biological evaluation of novel hydroxamic acid based histone deacetylase 6 selective inhibitors bearing phenylpyrazol scaffold as surface recognition motif (2017)4.Design, synthesis, 3D pharmacophore, QSAR, and docking studies of carboxylic acid derivatives as Histone Deacetylase inhibitors and cytotoxic agents (2014) 2. Discovery of a small molecule agonist of phosphatidylinositol 3-kinase p110α that reactivates latent HIV-1 (2014) 3. In Vivo Imaging of Histone Deacetylases (HDACs) in the Central Nervous System and Major Peripheral Organs (2014) 4. The effect of various zinc binding groups on inhibition of histone deacetylases 1-11 (2014) 5. Thermodynamics of ligand binding to histone deacetylase like amidohydrolase from Bordetella/Alcaligenes (2014) 6. A selective HDAC 1/2 inhibitor modulates chromatin and gene expression in brain and alters mouse behavior in two mood-related tests (2013) 7. Crebinostat: a novel cognitive enhancer that inhibits histone deacetylase activity and modulates chromatin-mediated neuroplasticity (2013) 8. FDG-PET imaging reveals local brain glucose utilization is altered by class I histone deacetylase inhibitors (2013) 9. Highly ligand efficient and selective N-2-(Thioethyl)picolinamide histone deacetylase inhibitors inspired by the natural product psammaplin A (2013) 10. Total synthesis and full histone deacetylase inhibitory profiling of Azumamides A-E as well as β²- epi-Azumamide E and β³-epi-Azumamide E (2013)

Warning(s): Avoid freeze/thaw cycles. Scientific Category: Deacetylase Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.

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