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UBB; Ubiquitin B

Protein

E. coli

Human ubiquitin (Accession Nr. P0CG47) coupled to agarose.

Human

Agarose

Liquid. In 20% Ethanol.

Use: Covalently coupled to agarose beads via primary amines, allowing for a fully functional C-terminus. Ideal for the enrichment of known ubiquitin-interacting proteins as well as the discovery of novel ubiquitin-interacting proteins. We recommend equilibrating the resin by washing with 5-10ml of your desired aqueous buffer.

Manufactured by Boston Biochem

BLUE ICE

+4°C

+4°C

Do not freeze.

Stable for at least 3 months after receipt when stored at +4°C.

No

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Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast ubiquitin, and 100% aa sequence identity shared between human and mouse ubiquitin. In mammals, four ubiquitin genes encode for two ubiquitin-ribosomal fusion proteins and two poly-ubiquitin proteins. Cleavage of the ubiquitin precursors by deubiquitinating enzymes gives rise to identical ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a ubiquitin-activating (E1) enzyme, a ubiquitin-conjugating (E2) enzyme, and a ubiquitin ligase (E3). ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting.

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