Background:JARID1C, also known as SMCX (Smcy homolog, X-linked), KDM5C, or XE169, is a JumonjiC (JmjC) and ARID domain-containing histone lysine demethylase that exhibits demethylation activity toward di- and trimethyl-lysine 4 (H3K4me2/3) on histone H3. Description:The JARID1C Homogeneous Assay Kit is designed to measure activity of the JARID1C for screening and profiling applications, using AlphaLISA^® technology. The key to the JARID1C Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, a sample containing JARID1C enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts. Synonym(s): JARID1C, SMCX, Smcy homolog, X-linked, KDM5C, XE169 Supplied As: The JARID1C Homogeneous Assay Kit comes in a convenient AlphaLISA^® format, with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JARID1C for 384 enzyme reactions. Contraindications: Avoid green and blue dyes that absorb light in the Alphascreen^® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN₃) or metal ions (Fe²⁺, Fe³⁺, Cu²⁺, Zn²⁺ and Ni²⁺). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen^® assays. Format: Instructions for use: See assay kit data sheet for detailed protocol. Storage / Stability: >6 months at –80°C Application(s): Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. Reference(s): Iwase S, Lan F, Bayliss P, et al. Cell 2007; 128(6):1077-1088 Notes:

The buffer in this kit was reformulated in May of 2015 with a different reducing agent to improve assay performance. The old formulation can still be purchased upon special request.

Warning(s): Avoid freeze/thaw cycles. Scientific Category: Demethylase Product Type: Assay Kit Data shown is lot-specific. Contact us for specific information on other lots.

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