Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。

Cataloguenumber	crb2005119
Antibody	Anti-ɣH2AX(pS139)antibody
AntigenPeptide	KLHconjugatedsyntheticpeptidecrb1200386e
ProteinID	UniProtKB-P16104
Aliases	PhosphorylatedHistoneH2AX(pS139)
Cross-Reactivity	Human,mouse
HostSpecies	Anti-Rabbit
AntibodyType	Polyclonal
Concentration	1mg/ml
Validation	1:1000WB,1:800IF,1:1000ELISA
Target	gammaH2AX(pS139)
DiseaseArea	Histones,DNAdamage
Family	Histones
Post-translationalModification	PhosphorylationofSerine139
Storage	ThismaterialissuppliedinPBScontaining0.01%sodiumazideand1%trehalose.Theproductshouldbestoredat+4°Cforshorttermstorageand-20°Cforlongtermstorage.Avoidrepeatedfreeze/thawcycles.
References	Feng,Y.,Xiang,J.,Liu,S.,Guo,T.,Yan,G.,Feng,Y.,Kong,N.,Li,H.,Huang,Y.,Lin,H.,Cai,X.andXie,A.(2017).H2AXfacilitatesclassicalnon-homologousendjoiningattheexpenseoflimitednucleotidelossatrepairjunctions.NucleicAcidsRes,45(18),10614-10633.PMID:28977657 KuoLJ1,YangLX.(2008).Gamma-H2AX-anovelbioMarkerforDNAdouble-strandbreaks.InVivo,22(3),305-9.PMID:18610740