Catalogue numbercrb2005083AntibodyAnti-V5 Epitope Tag antibodyAntigen PeptideKLH conjugated synthetic peptide crb1200007eAliasesV5 TagHost Species RabbitAntibody TypePolyclonalConcentration1mg/mlValidation1:2000 (WB), 1:1000 (ELISA)TargetV5 Epitope TagSpecificityProtein TagStorage This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.References

Cotter, K., Capecci, J., Sennoune, S., Huss, M., Maier, M., Martinez-Zaguilan, R. and Forgac, M. (2014). Activity of Plasma Membrane V-ATPases Is Critical for the Invasion of MDA-MB231 Breast Cancer Cells. J Biol Chem, 290(6), 3680-3692. PMID: 25505184

Ranawakage, D., Takada, T. and Kamachi, Y. (2019). HiBiT-qIP, HiBiT-based quantitative immunoprecipitation, facilitates the determination of antibody affinity under immunoprecipitation conditions. Sci Rep, 9(1). PMID: 31053795

Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。