Cataloguenumbercrb2005185AntibodyAnti-HistoneH3(G34R)antibodyAntigenPeptideKLHconjugatedsyntheticpeptidecrb1200376eProteinIDUniProtKB-P84243AliasesH3HistoneFamilyMember3A,H3Histone,H3.3AH3F3,HistoneH3.3,H3F3ACross-ReactivityHuman,Rabbit,MouseHostSpeciesRabbitAntibodyTypePolyclonalConcentration0.4mg/mlValidation1:1000(WB),1:1000(ELISA),IF,IHCTargetHistoneH3(H3.3)DiseaseAreaHistones,TumourigenesisFamilyHistonesSpecificityProteinStorageThismaterialissuppliedinPBScontaining0.01%sodiumazideand1%trehalose.Theproductshouldbestoredat+4°Cforshorttermstorageand-20°Cforlongtermstorage.Avoidrepeatedfreeze/thawcycles.CitationsHaqueetal.(2017)ActaNeuropatholCommun.5(1):45.PMID:28587626References

Voon,H.,Udugama,M.,Lin,W.,Hii,L.,Law,R.,Steer,D.,Das,P.,Mann,J.andWong,L.(2018).InhibitionofaK9/K36demethylasebyanH3.3pointmutationfoundinpaediatricglioblastoma.NatureCommun,9(1).PMID:30087349

Wu,G.,Broniscer,A.,McEachron,T.,Lu,C.,Paugh,B.,Becksfort,J.,Qu,C.,Ding,L.,Huether,R.,Parker,M.,Zhang,J.,Gajjar,A.,Dyer,M.,Mullighan,C.,Gilbertson,R.,Mardis,E.,Wilson,R.,Downing,J.,Ellison,D.,Zhang,J.andBaker,S.(2012).SomatichistoneH3alterationsinpediatricdiffuseintrinsicpontinegliomasandnon-brainstemglioblastomas.NatureGenet,44(3),251-253.PMID:22286216

Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。