品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
AlternativeNameLongneurotoxin1,α-Bgtx,α-BuTX
LyophilizedPowderLyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
BioassayTestedBioassayTested
Everylotistried&testedinarelevantBIOLOGicalassay.
OurBioassay
OriginModifiednaturalproteinisolatedfromBungarusmulticinctus (Many-bandedkrait).
MW:~9030Da.
Purity:>95%
FormLyophilizedpowder.
Effectiveconcentration100-200nM.
ModificationsDisulfidebondsbetweenCys3-Cys23,Cys16-Cys44,Cys29-Cys33,Cys48-CysandCys60-Cys65.
LabelLC-Biotin.Theextentoflabelingis1-3moleculesofbiotinpermoleculeα-Bungarotoxin.
Activityα-BungarotoxinblockspostsynapticneuromusculartransmissionviacompetitiveinhibitionofnicotinicAChreceptors(nAChRs),therebypreventingthedepolarizingactiononpostsynapticmembranesandblockingneuromusculartransmission.Selectiveforα7receptors(IC50 valueof1.6nM)andα3/β4receptors(IC50 valueof>3μM)1,2.ThetoxinalsoblocksGABA(A)receptorsubtypes3.
- Wilson,S.P.andKirshner,N. (1977) J.NeuRochem. 28,687.
- Garcia-Guzman,M. etal. (1995) Eur.J.Neurosci. 7, 647.
- McCann,C.M. etal. (2006) Proc.Natl.Acad.Sci.U.S.A. 103, 5149.
ShippingandstorageShippedatroomtemperature.Productassuppliedcanbestoredintactatroomtemperatureforseveralweeks.Forlongerperiods,itshouldbestoredat-20°C.
SolubilityAnyaqueousbuffer.Centrifugeallproductpreparationsbeforeuse(10000xg5min).
StorageofsolutionsUptotwoweeksat4°Corthreemonthsat-20°C.
Bungarotoxinbindingsitesco-localizewithGABAergicneuronsexpressingparvalbumininmousehippocampalCA1region.A.Freefloatingmousebrainsectionswereincubatedwith α-Bungarotoxin-Biotin (#B-100-B),(1:10,000)followedbystreptavidin-Alexa488(green).B.Samesectionswerestainedwithanti-parvalbumin,followedbygoatanti-mouselabeledwithTexasredisothiocyanate(TRITC).C.MergeofAandBdemonstratessitesofcolocalization(verticalarrows)confirmingreportsthatasubsetofhippocampalinterneuronsexpressnAChRα7.DAPIisusedasthecounterstain.
AlomoneLabsα-Bungarotoxin-BiotininhibitsmusclenAChRchannelsheterologouslyexpressedin Xenopus oocytes.A.TimecourseofmusclenAChR(α1/β1/γ/δ)currentrecording.Membranepotentialwasheldat-80mVandstimulatedbyperfusedwithasolutioncontaining100µMAChand3μMPNU-120596every100sec.200nM α-Bungarotoxin-Biotin (#B-100-B)wasapplied(green)duringtheAChapplicationfor5.5minandinhibitedchannelcurrent,asindicated.B.SuperimposedexamplesofmusclenAChRcurrentintheabsence(black)andpresence(green)of200nMα-Bungarotoxin-Biotin(takenfromtheexperimentinA).
References-Scientificbackground
- 1.Ohta,M. etal. (1987) FEBSLett. 222, 79.
- 2.Wilson,P.T. etal. (1988) Mol.Pharmacol. 34, 643.
- 3.Wilson,S.P.andKirshner,N. (1977) J.Neurochem. 28,687.
- 4.Garcia-Guzman,M. etal. (1995) Eur.J.Neurosci. 7, 647.
- 5.McCann,C.M. etal. (2006) Proc.Natl.Acad.Sci.U.S.A. 103, 5149.
α-BungarotoxinisoformA31isa74aminoacidpeptidyltoxinisolatedfromthevenomofthebandedkraitsnake, Bungarusmulticinctus1.
α-BungarotoxinblockspostsynapticneuromusculartransmissionviacompetitiveinhibitionofnicotinicAChreceptors(nAChRs)withanIC50 of3.5x10-10 M,therebypreventingthedepolarizingactiononpostsynapticmembranesandblockingneuromusculartransmission2.
Thetoxinisselectivefor α7 receptors(IC50 valueof1.6nM)and α3/β4 receptors(IC50 valueof>3µM)3,4.
α-Bungarotoxinalsobindstoandblocksasubsetof GABAA receptors (GABAARs)thatcontaintheGABAARβ3subunit.Inparticular,α-BungarotoxinblocksGABAARsthatcontaininterfacesbetweenadjacentβ3subunits5.
Targetα7,α1/β1/γ/δnAChR,GABA(A)receptorsubtypes
NetPeptideContent:100%
LyophilizedPowder
Lastupdate:04/11/2019
α-Bungarotoxin-Biotin(#B-100-B)isahighlypure,natural,andbiologicallyactiveconjugatedpeptidetoxin.
Wegladlytakeoncollaborationprojects.PleaseContactUs.
Forresearchpurposesonly,notforhumanuse
Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。
