Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Negative control antigen reconstitution 100 µl double distilled water (DDW).
Negative control antigen storage after reconstitution -20°C.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5), U-87 MG (lanes 3 and 6) and C6 (lanes 4 and 8) lysates:
1. Anti-CALHM1 Antibody (#ACC-101), (1:200). 2. Anti-CALHM1 Antibody, preincubated with the negative control antigen.
Expression of CALHM1 in rat hippocampus
Immunohistochemical staining of rat hippocampus using Anti-CALHM1 Antibody (#ACC-101). A. Staining of CALHM1 appears only in the hippocampal CA1 pyramidal layer (red). B. Nuclear staining using DAPI as the counterstain (blue). C. Merge of A and B.
References
1. Green, K.N. and LaFerla, F.M. (2008) Neuron59, 190.
2. Koppel, J. et al. (2011) Mol. Med.17, 974.
3. Dreses-Werringloer, U. et al. (2008) Cell133, 1149.
A misbalance in Ca2+ homeostasis seems to be related to the development of Alzheimer’s disease (AD). Indeed, neuronal Ca2+ balance may affect the levels of proteins associated with AD, such as amyloid-beta (Ab) and tau1.
Calcium homeostasis modulator 1 (CALHM1) is a cerebral Ca2+ channel component responsible for controlling intracellular Ca2+ levels and Ab metabolism2,3. CALHM1 is a three transmembrane glycoprotein, mostly localized to the endoplasmic reticulum although it can be detected at the plasma membrane. A functional channel is formed by multiple subunits which has some structural similarities to the NMDA receptor. It is expressed in all brain regions1.
In cultured cells, overexpression of CALHM1 increases intracellular Ca2+ levels and reduces Ab accumulation, thereby reinforcing its implication in the development of AD. Indeed, a polymorphism in the gene was found to affect the onset of AD2.
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Lyophilized PowderNegative Control Antigen Included
Click here to receive a 50 µl free trial sample!
Certificate of Analysis MSDS
Last update: 10/11/2019
Anti-CALHM1 Antibody (#ACC-101) is a highly specific antibody directed against an epitope of the human calcium homeostasis modulator protein 1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CALHM1 from rat, mouse and human samples.
For research purposes only, not for human use
Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。
Lyophilized Powder 
Antigen Incl. 
Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5), U-87 MG (lanes 3 and 6) and C6 (lanes 4 and 8) lysates:1. Anti-CALHM1 Antibody (#ACC-101), (1:200).
Expression of CALHM1 in rat hippocampusImmunohistochemical staining of rat hippocampus using Anti-CALHM1 Antibody (#ACC-101). A. Staining of CALHM1 appears only in the hippocampal CA1 pyramidal layer (red). B. Nuclear staining using DAPI as the counterstain (blue). C. Merge of A and B.
