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蚂蚁淘在线 / 品牌中心 / Discovery / Discovery Antibodies/Anti-CACNA1G (CaV3.1)-ATTO-594 Antibody/50 µl/ACC-021-AR-50 µl

Discovery Antibodies/Anti-CACNA1G (CaV3.1)-ATTO-594 Antibody/50 µl/ACC-021-AR-50 µl

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￥10540.00

货号：ACC-021-AR-50µl

浏览量：127

品牌：Discovery

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商品描述

Cat#:ACC-063-AO

AlternativeNameStromalinteractionmolecule1,GOK

LyophilizedPowder

AntigenIncl.

Type:Polyclonal

Host:Rabbit

Reactivity:h,m,r

Immunogen-+

Accession(Uniprot)NumberQ13586

GeneID6786

PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.

HomologyRat,mouse,bovineandcanis-identical.

RRIDAB_2039894.

PurityAffinitypurifiedonimmobilizedantigen.

FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN₃.

IsotypeRabbitIgG.

LabelATTO-550.Maximumabsorption554nm;maximumfluorescence576nm.Thefluorescenceisexcitedmostefficientlyinthe540-565nmrange.ThislabelisrelatedtothedyeRhodamine6GandcanbeusedwithfiltersusedtodetectRhodamine.

StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.

Reconstitution50µldoubledistilledwater(DDW).

Antibodyconcentrationafterreconstitution1mg/ml.

StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).

NegativecontrolantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.

Negativecontrolantigenreconstitution100µldoubledistilledwater(DDW).

Negativecontrolantigenstorageafterreconstitution-20°C.

PreadsorptionControl10μgpeptideper1μgantibody.

StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#ACC-063),andimmunohistochemistry(labeledantibody).

Applications:ic,if,ih,lci

Immunohistochemistry-+

Livecellimaging/Immunocytochemistry-+

References

1.Eisner,D.A.etal.(2005)Exp.Physiol.90,3.

2.Chakrabarti,R.andChakrabarti,R.(2006)J.Cell.Biochem.99,1503.

3.Feske,S.etal.(2006)Nature441,179.

4.Pickett,J.(2006)NatureReviewsMol.CellBiol.7,794.

5.Dziadek,M.A.andJohnstone,L.S.(2007)CellCalcium42,123.

6.Yuan,J.P.etal.(2007)Nat.CellBiol.9,636.

7.Muik,M.etal.(2008)J.Biol.Chem.283,8014.

8.Li,Z.etal(2007)J.Biol.Chem.282,29448.

9.Hewavitharana,T.etal.(2007)CellCalcium42,173.

Scientificbackground-+

LyophilizedPowderNegativeControlAntigenIncluded

Lastupdate:10/11/2019

Anti-STIM1(extracellular)Antibody(#ACC-063)isahighlyspecificantibodydirectedagainstanextracellularepitopeofthehumanStromalinteractionmolecule1.Theantibodycanbeusedinwesternblot,immunocytochemistry,immunohistochemistry,andindirectflowcytometryapplications.IthasbeendesignedtorecognizeSTIM1fromhuman,rat,andmousesamples.

Anti-STIM1(extracellular)-ATTO-550Antibody(#ACC-063-AO)isdirectlylabeledwithanATTO-550fluorescentdye.ATTOdyesarecharacterizedbystrongabsorption(highextinctioncoefficient),highfluorescencequantumyield,andhighphoto-stABIlity.TheATTO-550fluorescentlabelisrelatedtothewellknowndyeRhodamine6GandcanbeusedwithfilterstypicallyusedtodetectRhodamine.Anti-STIM1(extracellular)-ATTO-550AntibodyhasbeentestedinimmunocytochemistryandimmunohistochemistryapplicationsandisespeciallysuitedforexperimentsrequiringsimultaneouslabelingofdifferentMarkers.

Forresearchpurposesonly,notforhumanuse

Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。

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Discovery Antibodies/Anti-CACNA1G (CaV3.1)-ATTO-594 Antibody/50 µl/ACC-021-AR-50 µl

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