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蚂蚁淘在线 / 品牌中心 / Discovery / Discovery Antibodies/Anti-α1-Syntrophin (SNTA1) Antibody/25 µl/APZ-021-25 µl

Discovery Antibodies/Anti-α1-Syntrophin (SNTA1) Antibody/25 µl/APZ-021-25 µl

价格
￥4940.00
货号：APZ-021-25µl
浏览量：51
品牌：Discovery
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商品描述
Cat#:APZ-021
AlternativeNameSyntrophinalpha1,Syntrophin-1,59kDadystrophin-associatedproteinA1acidiccomponent1
  • LyophilizedPowder
  • AntigenIncl.
    • Type:Polyclonal
    Host:Rabbit
    Reactivity:h,m,r
      • Peptide(C)RQPSSPGPQPRNLSE,correspondingtoaminoacidresidues191-205ofmouseα1-syntrophin(Accession Q61234).Intracellular,PH1domain.
        Anti-α1-Syntrophin (SNTA1) Antibody
    Accession(Uniprot)NumberQ61234
    GeneID20648
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyMouse,human–identical.
    RRIDAB_2756776.
    PurityAffinitypurifiedonimmobilizedantigen.
    FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    IsotypeRabbitIgG.
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
    Antibodyconcentrationafterreconstitution0.8mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
    NegativecontrolantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
    Negativecontrolantigenreconstitution100µldoubledistilledwater(DDW).
    Negativecontrolantigenstorageafterreconstitution-20°C.
    PreadsorptionControl1µgpeptideper1µgantibody.
    StandardqualitycontrolofeachlotWesternblotanalysis.
    Applications:if,ih,wb
    Mayalsoworkin:ic*,ifc*,ip*
      • Anti-α1-Syntrophin (SNTA1) Antibody
        Westernblotanalysisofmouseheartlysate(lanes1and3)andratskeletalmusclemembranes(lanes2and4):
        1,2. Anti-α1-Syntrophin(SNTA1)Antibody (#APZ-021),(1:400).
        3,4.Anti-α1-Syntrophin(SNTA1)Antibody,preincubatedwiththenegativecontrolantigen.
        Anti-α1-Syntrophin (SNTA1) Antibody
        Westernblotanalysisofmousebrainlysate:
        1. Anti-α1-Syntrophin(SNTA1)Antibody (#APZ-021),(1:200).
        2.Anti-α1-Syntrophin(SNTA1)Antibody,preincubatedwiththenegativecontrolantigen.
      • Anti-α1-Syntrophin (SNTA1) Antibody
        ExpressionofSyntrophinalpha1inmouseneocortex
        Immunohistochemicalstainingofperfusion-fixedfrozenmousebrainsectionsusingAnti-α1-Syntrophin(SNTA1)Antibody (#APZ-021),(1:400),followedbyanti-rabbit-AlexaFluor-488antibody(green).Syntrophinalpha1staining(green)appearsinbloodvessels(horizontalarrows)andinthepiamater(verticalarrows).NucleiarestainedwithDAPI(blue).
    References
    • 1.Bhat,H.F.etal.(2014)Br.J.Cancer.110,706.
    • 2.Wilde,A.A.etal.(2011)Circ.Res.108,884.
    • 3.Willis,B.C.etal.(2105)Am.J.Physiol.Heart.Circ.Physiol.308,1463.
    • 4.Anderova,M.etal.(2014)PLoSOne9,e113444.
    • 5.Williams,J.C.etal.(006)J.Biol.Chem.281,23341.
    • 6.Koo,T.etal.(2011)Hum.Gene.Ther.22,1379.

      • α1-Syntrophinisacomponentofthedystrophinscaffold-proteincomplexthatactsinrecruitingproteinsthroughtheirmultipleprotein–proteininteractionmotifstothecytoplasmicsideofplasmamembranes.Proteinsinteractingwithα1-syntrophinincludevoltage-gatedsodiumchannels,neuronalnitricoxidesynthase,calmodulin,ankyrinrepeat-richmembranespanningprotein,G-proteinsandmore. 

        Thesyntrophinfamilycomprisesfivemembers:α1-syntrophin,β1-syntrophin,β2-syntrophin,γ1-syntrophin,andγ2-syntrophin1.

        α1-Syntrophinisaperipheralcytoplasmicmembraneproteininskeletalandcardiacmusclecells1.

        Theα1-syntrophinstructurecontainsfourconserveddomains,twopleckstrinhomologydomains(PH1andPH2),aPSD95-diskslarge-ZO1(PDZ)domainandasyntrophinunique(SU)COOH-terminaldomain2.ThepleckstrindomainsareresponsIBLefortherecruitmentofproteinstothesarcolemma.ThePDZdomainisinsertedwithinthePH1domainandbindstoNOS-1inskeletalmuscle.ThisdomainalsobindstotheC-terminusofthe Kir2.1 channelandalsoto NaV1.5 andsoallowingthemtointeract.TheSUdomainbindssyntrophintodystrophin2,3.α1-Syntrophinisalsoresponsibleforthesite-specificanchoringof AQP4 protein.α1-SyntrophindeletionleadstothedisruptionofthepolarizedsubcellularexpressionofAQP4intheperivascularmembranes4. 

        Severalstudieshaveshownthatsyntrophin-deficiencyinDuchennemusculardystrophycanultimatelyleadtomuscledegeneration.Syntrophin-deficiencywasalsoshowntoreducelevelsofnNOSinmice5,6.

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IF-Immunofluorescence,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    LyophilizedPowderNegativeControlAntigenIncluded
    receivea25µlfreetrialsample!
    Image&Title:
    Anti-α1-Syntrophin (SNTA1) Antibody
    Immuno-colocalizationofKir4.1andα1-Syntrophininratfornix
    Immunohistochemicalstainingofimmersion-fixed,freefloatingratbrainfrozensectionsusingGuineapigAnti-Kir4.1(KCNJ10)Antibody(#AGP-012),(1:400)andrabbitAnti-α1-Syntrophin(SNTA1)Antibody (#APZ-021),(1:300).A.Kir4.1staining(red)appearsinbloodvesselprofiles(arrows)inthefornix.B.Syntrophinalpha1staininginthesamesection(green)appearsinseveralelementsincludingbloodvessels(arrows).C.Mergeofthetwoimagesrevealspartialco-localizationofKir4.1andsyntrophinalpha1inhilarcells.CellnucleiarestainedwithDAPI(blue).
    Lastupdate:10/11/2019

    AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanepitopeofmousesyntrophinalpha1.Anti-α1-Syntrophin(SNTA1)Antibody(#APZ-021)canbeusedinwesternblotandimmunohistochemistryapplications.Ithasbeendesignedtorecognizesyntrophinalpha1frommouse,ratandhumansamples.

    Forresearchpurposesonly,notforhumanuse
    Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。

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