AlternativeName Voltage-dependentT-typecalciumchannelsubunitα1G
KOValidated KOValidated
Thespecificityofthisantibodyhasbeenvalidatedinaknockout(KO)orknockdown(KD)system.
LyophilizedPowder LyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl. AntigenIncl.
ThisantibodyisshippedwithitsantigenFREEofcharge!
Type: Polyclonal
Host: Rabbit
Reactivity: h,m,r
Immunogen- + PeptideMDEEEDGAGAEESGQPRSFTQL(C),correspondingtoaminoacidresidues1-22ofratCACNA1G (Accession O54898).Intracellular,N-terminus.
Accession(Uniprot)Number O54898
Peptideconfirmation Confirmedbyaminoacidanalysisandmassspectrometry.
Homology Mouse -identical;human-20/22aminoacidresiduesidentical.
RRID AB_2340896.
Purity Affinitypurifiedonimmobilizedantigen.
Form Lyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3 .
Isotype RabbitIgG.
Label ATTO-594.Maximumabsorption601nm;maximumfluorescence627nm.Thefluorescenceisexcitedmostefficientlyinthe580-615nmrange.ThislabelisrelatedtotheRhodaminedyesandcanbeusedwithfiltersusedtodetectTexasRedandAlexa-594.
Storagebeforereconstitution Theantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
Reconstitution 50µldoubledistilledwater(DDW).
Antibodyconcentrationafterreconstitution 1mg/ml.
Storageafterreconstitution Thereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).
Negativecontrolantigenstoragebeforereconstitution Lyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
Negativecontrolantigenreconstitution 100µldoubledistilledwater(DDW).
Negativecontrolantigenstorageafterreconstitution -20°C.
PreadsorptionControl 1µgpeptideper1µgantibody.
Standardqualitycontrolofeachlot Westernblotanalysis(unlabeledantibody,#ACC-021),andimmunohistochemistry(labeledantibody).
Applications: if,ih
Mayalsoworkin: ic*
Immunohistochemistry- + ExpressionofCACNA1G inratandmousecerebellum
Immunohistochemicalstainingofmouse(A)andrat(B)cerebellumusing Anti-CACNA1G(CaV 3.1)-ATTO-594 Antibody (#ACC-021-AR).A,B.CACNA1G staining(red)appearsinPurkinjeneurons(arrow)andinthemolecularlayer(Mol).NucleiwerestainedusingDAPIasthecounterstain(Blue).
References
1.Serrano,J.R.etal. (1999)J.Gen.Physiol. 114, 185. 2.Perez-Reyes,E.(2003)Physiol.Rev. 83, 117. 3.Wain,H.M.etal. (2002)NucleicAcidsRes. 30, 169. 4.Andreasen,D.etal. (2000)Am.J.Physiol. 279, F997. Scientificbackground- + Voltage-dependentCa2+ channelsprovideapathwayforrapidinfluxofCa2+ intocells, whichplaysacrucialroleinbothelectricalandmetabolicsignaling.1
T-typecurrentsaretransducedviachannelproteinsencodedbythreegenesthat composeasubfamilywithintheCaV channelfamily.2-3
TheactivityofT-typechannelscontributestoseveralknownphysiologicalandpathophysiological phenomenaincludingburstfiringinneurons,pacemakingactivityintheheartandsecretionfromendocrinetissues.2 TherearethreeclonedT-typechannelisoforms.
CACNA1G(CaV 3.1)andCACNA1H(CaV 3.2)arewidelydistributedwhereastheexpressionofCACNA1I(CaV 3.3) isrestrictedtothecentralnervoussystem.2
CACNA1GandCACNA1H arealsoexpressedinthekidney,butlittleisknownabouttheirphysiologicalrolethere.
LyophilizedPowder NegativeControlAntigenIncluded
Lastupdate:10/11/2019
Anti-CACNA1G(CaV 3.1)Antibody(#ACC-021)isahighlyspecificantibodydirectedagainstanepitopeoftheratCaV 3.1channel.Theantibody canbeusedinwesternblot,immunocytochemistry,andimmunohistochemistryapplications.IthasbeendesignedtorecognizeCACNA1G fromhuman,rat,andmousesamples.
Anti-CACNA1G(CaV 3.1)-ATTO-594Antibody (#ACC-021-AR)isdirectlylabeledwithATTO-594fluorescentdye.ATTOdyesarecharacterizedbystrongabsorption(highextinctioncoefficient),highfluorescencequantumyield,andhighphoto-stABI V 3.1)-ATTO-594Antibodyisidealforimmuno-colocalizationstudies.
Forresearchpurposesonly,notforhumanuse
Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。
KOValidated
LyophilizedPowder
AntigenIncl.
ExpressionofCACNA1G inratandmousecerebellumImmunohistochemicalstainingofmouse(A)andrat(B)cerebellumusing Anti-CACNA1G(CaV3.1)-ATTO-594 Antibody(#ACC-021-AR).A,B.CACNA1G staining(red)appearsinPurkinjeneurons(arrow)andinthemolecularlayer(Mol).NucleiwerestainedusingDAPIasthecounterstain(Blue).
