AlternativeName G-proteincoupledbileacidreceptor1,M-BAR,GPR131,BG37
LyophilizedPowder LyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl. AntigenIncl.
ThisantibodyisshippedwithitsantigenFREEofcharge!
Type: Polyclonal
Host: Rabbit
Reactivity: m,r
Mayalsoworkin: h*
Immunogen- + Peptide(C)GDQRYTAPWRTAAQR,correspondingtoaminoacidresidues282-296ofratGPBAR1(Accession Q80T02).Intracellular,C-terminus.
Accession(Uniprot)Number Q80T02
GeneID 7434
Peptideconfirmation Confirmedbyaminoacidanalysisandmassspectrometry.
Homology Mouse-identical;human-14/15aminoacidresiduesidentical.
RRID AB_2340892.
Purity Affinitypurifiedonimmobilizedantigen.
Form Lyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3 .
Isotype RabbitIgG.
Storagebeforereconstitution Theantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
Reconstitution 25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
Antibodyconcentrationafterreconstitution 0.8mg/ml.
Storageafterreconstitution Thereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
Negativecontrolantigenstoragebeforereconstitution Lyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
Negativecontrolantigenreconstitution 100µldoubledistilledwater(DDW).
Negativecontrolantigenstorageafterreconstitution -20°C.
PreadsorptionControl 2µgpeptideper1µgantibody.
Standardqualitycontrolofeachlot Westernblotanalysis.
Applications: wb
Mayalsoworkin: ic* ,ifc* ,ih* ,ip*
Westernblot- + Westernblotanalysisofratbrain(lanes1and4),mouseliver(lanes2and5)andratspleen(lanes3and6) lysates :
1-3. Anti-TGR5(GPBAR1)Antibody (#ABR-031),(1:200).
References
1.Duboca,H.etal .(2014)Dig.LiverDis. 46, 302. 2.Hov,J.R.etal .(2010)PLoSONE 5, e12403. 3.Kawamata,Y.etal .(2003)J.Biol.Chem. 278, 9435. 4.Chen,X.andDing,H.(2011)Exp.DiabetesRes. 2011, 853501. 5.Pols,T.W.etal. (2011)J.Hepatol. 54, 1263. Scientificbackground- + BileacidsareendogenousligandsforTGR5,a G-proteincoupledreceptor.Bileacidsareimportantamphipathicbiomoleculeswhichcanhelpabsorptionofdietarylipidsandfat-solublevitaminsintheintestine.TheTGR5receptoristhefirstknownG-proteincoupledreceptorspecificforbileacids1 .
ThecodingsequenceoftheTGR5genecontains993basepairs,encodingaproteinof330aminoacids.Thereceptorcomprisesseventransmembranehelices,threeextracellularloopscontributingtoligandbinding,andthreeintracellularloopsinvolvedinmediatingthesignaltodownstreamsignalingmolecules2 .
TGR5geneexpressioniswidelydistributed,includingendocrineglands,ADI 3 .
AnimalstudiessuggestthatTGR5activationinfluencesenergyproductionandtherebymaybeinvolvedinobesityanddiabetes.TGR5activationalsoinfluencesintestinalmotility.TGR5isnowrecognizedasapotentialtargetforthetreatmentofmetabolicdisorders,suchastype2diabetes1 .Inaddition,theABI 4,5 .
LyophilizedPowder NegativeControlAntigenIncluded
Clickhereto receivea25µl freetrialsample!
Lastupdate:19/08/2019
Alomone Anti-TGR5(GPBAR1)Antibody (#ABR-031)canbeusedinwesternblotanalysis.IthasbeendesignedtorecognizeTGR5frommouse,ratandhumansamples.
Forresearchpurposesonly,notforhumanuse
Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。
LyophilizedPowder
AntigenIncl.
Westernblotanalysisofratbrain(lanes1and4),mouseliver(lanes2and5)andratspleen(lanes3and6) lysates:1-3. Anti-TGR5(GPBAR1)Antibody (#ABR-031),(1:200).
