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蚂蚁淘在线 / 品牌中心 / Discovery / Discovery Antibodies/Anti-B1 Bradykinin Receptor (BDKRB1) Antibody/25 µl/ABR-011-25 µl

Discovery Antibodies/Anti-B1 Bradykinin Receptor (BDKRB1) Antibody/25 µl/ABR-011-25 µl

价格
￥4940.00
货号：ABR-011-25µl
浏览量：127
品牌：Discovery
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商品描述
Cat#:ABR-011
AlternativeNameB1R,BKR1,B1BKR,BKRB1, KininB1,BRADYB1
  • LyophilizedPowder
  • AntigenIncl.
    • Type:Polyclonal
    Host:Rabbit
    Reactivity:m,r
      • Peptide(C)KEASRTR*SGGPKGSK,correspondingtoaminoacidresidues243-257ofratB1Rwithreplacementofcysteine250(C250)withserine(*S)(Accession P97583).3rd intracellularloop.
        Anti-B1 Bradykinin Receptor (BDKRB1) Antibody
    Accession(Uniprot)NumberP97583
    GeneID81509
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyMouse-13/15aminoacidresiduesidentical.
    RRIDAB_2039752.
    PurityAffinitypurifiedonimmobilizedantigen.
    FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    IsotypeRabbitIgG.
    SpecificityMightnotrecognizethehumanepitope.
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
    Antibodyconcentrationafterreconstitution0.8mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
    NegativecontrolantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
    Negativecontrolantigenreconstitution100µldoubledistilledwater(DDW).
    Negativecontrolantigenstorageafterreconstitution-20°C.
    PreadsorptionControl1µgpeptideper1µgantibody.
    StandardqualitycontrolofeachlotWesternblotanalysis.
    Applications:ic,if,ih,wb
    Mayalsoworkin:ifc*,ip*
      • Anti-B1 Bradykinin Receptor (BDKRB1) Antibody
        Westernblotanalysisofratbrain(lanes1and3)andheart(lanes2and4)lysates:
        1. Anti-B1 BradykininReceptor(BDKRB1)Antibody(#ABR-011),(1:200).
        2.Anti-B1 BradykininReceptor(BDKRB1)Antibody,preincubatedwiththenegativecontrolantigen.
      • Anti-B1 Bradykinin Receptor (BDKRB1) Antibody
        ExpressionofB1 bradykininreceptor inratCNS
        Immunohistochemicalstainingoffrozenratbrain section(A&B)andcerebellum(C)using Anti-B1 BradykininReceptor(BDKRB1)Antibody(#ABR-011),(1:50).BKR1 isexpressedinneuronalcellsoftheamygdala(A)andhippocampus(CA1)(B)aswellasintheaxonsofPurkinjecellsinthecerebellum(arrowheadsonC).Hoechst33342isusedasthecounterstain.
        Mousespinalcord(1:200),(Dutra,R.C. etal. (2013) Neurobiol.Dis. 54, 82.).
      • Rattrigeminalneuronprimarycultures(Kawaguchi,A. etal. (2015) Front.Cell.Neurosci. 9, 229.).
    References
    • 1.Walker,K.etal.(1995)NeuRochem.Int.26,1.
    • 2.Böckmann,S.andPaegelow,I.(2000)J.Leukoc.Biol.68,587.
    • 3.Hess,J.F.etal.(2004)J.Pharmacol.Exp.Ther.310,488.

      • Kininsaresmallpeptidesrapidlyproducedfollowingtissueinjurythatserveasimportantmodulatorsofinflammationandpain.Intheperiphery,theactionsofkininsincludevasodilatation,increasedvascularpermeABIlity,stimulationofimmunecells,andinductionofpain.Kininsinthecentralnervoussystem(CNS)appeartoinitiateasimilarcascadeofeventsleADIngtoneuraltissuedamageaswellaslonglastingdisturbancesaffectingblood-brainbarrierfunction.1

        KininssuchasBradykinin(BK),Lys-BK,desArg9-BK,andLys-desArg9-BKexerttheiractionviatwodistinctreceptors:theB1 Bradykininreceptor(BKRB1)andthe B2 Bradykininreceptor (BKRB2).ThedesArg9-BKandLys-desArg9-BKpeptidesactivateBKRB1whileBKandLys-BKoperatebyactivatingBKRB2.2 BothBKRB1andBKRB2aremembersoftheseven-transmembranedomain,Gprotein-coupledreceptor(GPCR)superfamilyandshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,andacytoplasmiccarboxylterminus.

        ExpressionofBKRB1isinducIBLeuponvarioustypesoftissueinjuryandbyinflammatorymediatorssuchasbacteriallipopolysaccharide(LPS)andcytokines.BKRB1waslongconsiderednottobeexpressedinhealthytissues.However,recentworkhasdemonstratedalowlevelofexpressionofBKRB1intheCNSofrodentandprimates.Incontrast,BKRB2isconstitutivelyexpressedonvariouscelltypes.3 BKRB1representsapotentialtherapeutictargetfortreatmentofinflammatorydisordersandcardiovasculardiseases.

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,
    IF-Immunofluorescence,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    LyophilizedPowderNegativeControlAntigenIncluded
    receivea25µlfreetrialsample!
    Image&Title:

    Anti-B1 Bradykinin Receptor (BDKRB1) Antibody
    ExpressionofB1 bradykininreceptorinratTGneurons.Immunocytochemicalstainingofratprimarytrigeminalganglion(TG)neuronsusing Anti-B1 BradykininReceptor (BDKRB1) Antibody(#ABR-011). Adaptedfrom Kawaguchi,A. etal. (2015) Front.Cell.Neurosci. 9, 229.withpermissionofFrontiers.

    Lastupdate:10/11/2019

    Anti-B1BradykininReceptor(BDKRB1)Antibody(#ABR-011)isahighlyspecificantibodydirectedagainstanepitopeoftheratprotein.Theantibodycanbeusedinwesternblot,immunohistochemistry,andimmunocytochemistryapplications.IthasbeendesignedtorecognizeB1R fromratandmousesamples.Theantibodymaynotrecognizethereceptorfromhumansamples.

    Forresearchpurposesonly,notforhumanuse
    Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。

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