Lyophilized Powder This product is freeze dried. All water molecules have been removed.
Bioassay Tested Every lot is tried & tested in a relevant biological assay.
Our Bioassay
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- Liao, C.F. et al. (1989) J. Biol. Chem. 264, 7328.
- Lazareno, S. et al. (1990) Br. J. Pharmacol. 109, 1120.
- Hernandez, M. et al. (1993) Br. J. Pharmacol. 110, 1413.
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Alomone Labs Tropicamide inhibits carbachol’s effect on M1 mAChR expressed in C6 cells.Cells were loaded with Fluo-3 AM and changes in intracellular Ca2+ were detected via changes in Fluo-3 emission. A. Normalized fluorescence before and after application (at 20 seconds, see arrow) of 10 μM Carbachol following a 20 min incubation with 0-100 μM Tropicamide (#T-120) (as indicated). B. Inhibition (as percent of control) of 10 µM Carbachol evoked Ca2+ rise, plotted against Tropicamide concentrations.
- 1. Bonner, T.I. et al. (1989) Trends. Neurosci. 12, 148.
- 2. Felder, C.C. et al. (1995) FASEB. J. 9, 619.
- 3. Bonner, T.I. et al. (1987) Science 237, 527.
- 4. Peralta, E.G. et al. (1987) EMBO J. 6, 3923.
- 5. Bonner, T.I. et al. (1988) Neuron 1, 403.
- 6. Liao, C.F. et al. (1989) J. Biol. Chem. 264, 7328.
- 7. Liao, C.F. et al. (1989) J. Biol. Chem. 264, 7328.
- 8. Lazareno, S. et al. (1990) Br. J. Pharmacol. 109, 1120.
- 9. Hernandez, M. et al. (1993) Br. J. Pharmacol. 110, 1413.
- 10. Manny, R.E. et al. (2001) Invest. Ophtalmol. Vis. Sci. 42, 1728.
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Muscarinic acetylcholine receptors (mAChR) regulate a number of important basic physiologic functions including heart rate, motor and sensory control and more complex behaviors including arousal, memory, and learning. Loss of muscarinic receptor number or function has been implicated in the etiology of several neurological disorders including Alzheimer"s dementia, Down"s syndrome and Parkinson"s disease.
Five subtypes of muscarinic receptors (m1-m5) have been identified by molecular cloning1 and much has been learned about their distribution, pharmacology, and structure2-6.
Tropicamide is a muscarinic receptor antagonist, which shows a 3 fold selectivity for m47-8. Its effect has a pIC50 of ~7.5 for porcine intravesical ureter contraction9.
Tropicamide produces short acting mydriasis (dilation of the pupil) and cycloplegia (paralysis of the ciliary muscle) when applied as eye drops. It is also an effective cycloplegic agent in myopic children10.
Tropicamide (#T-120) is a highly pure, synthetic, and biologically active compound.
