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商品描述
LyophilizedPowderLyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
BioassayTestedBioassayTested
Everylotistried&testedinarelevantBIOLOGicalassay.
OurBioassay
SourceSynthetic
MW:356.3
Purity:>99%(HPLC)
Effectiveconcentration1-5μM.
Chemicalname1,4-Dihydro-2,6-dimethyl-5-nitro-4-[2-(trifluoromethyl)phenyl]-3-pyridinecarboxylicacid,methylester.
MolecularformulaC16H15F3N2O4.
CASNo.:71145-03-4,93468-89-4.
Activity(±)-BayK8644isadihydropyridinethatactsasanL-type,voltage-gatedCa2+ channelagoNIST.The(-)-enantiomerhasstrongCa2+ channelagonistproperties,whereasthe(+)-enantiomerhasweakCa2+ channelantagonistactivity.ThenetactivityoftheracemicmixisL-typechannelopening.Inthepresenceofthisagonist,channelstendtoopenforlongerperiodscausingalargeincreaseinthechannelmicroscopicresponse1-4.(±)-BayK8644isalsoaninhibitor(byincreasingtheinactivation)ofa Shaker KV channel5.
- Hess,P. etal. (1984) Nature 311, 538.
- Nowycky,M.C. etal. (1985) Proc.Natl.Acad.Sci.U.S.A. 82, 2178.
- Bechem,M.andHoffmann,H. (1993) PflugersArch. 424, 343.
- Triggle,D.J.andRampe,D. (1989) TrendsPharmacol.Sci. 10, 507.
- Avdonin,V. etal. (1997) J.Gen.Physiol. 109, 169.
ShippingandstorageShippedatroomtemperature.Productassuppliedcanbestoredintactatroomtemperatureforseveralweeks.Forlongerperiods,itshouldbestoredat-20°C.
SolubilityEthanol,methanolorDMSO.Centrifugeallproductpreparationsbeforeuse(10000xg5min).
StorageofsolutionsUptofourweeksat4°Corthreemonthsat-20°C.
AlomoneLabs(±)-BayK8644increasesL-typeCa2+ currentsexpressedin Xenopus oocytes.Left:I-Vrelationbefore(squares)andduring(circles)bathperfusionof5μM (±)-BayK8644 (#B-350).Right:Anexampleofcurrentresponseto200msdepolarizationto+20mV(fromholdingpotentialof-100mV)before(black)andduring(pink)perfusionof(±)-BayK8644.
AlomoneLabs(±)-BayK8644modulatesthephosphorylationofERK1/2MAPKinJurkatTcells.CellswerepreincubatedwithorwithoutEGTAorNifedipinefor20minthenstimulatedwith (±)-BayK8644 (#B-350)orwith Ionomycin for10min.Thecellproteinswereblottedandprobedwithanti-phosphoERK1/2(upperpanel)orwithanti-ERK1/2(lowerpanel).
References-Scientificbackground
- 1.Hess,P. etal. (1984) Nature 311, 538.
- 2.Nowycky,M.C. etal. (1985) Proc.Natl.Acad.Sci. U.S.A. 82, 2178.
- 3.Bechem,M.andHoffmann,H. (1993) PflugersArch. 424, 343.
(±)-BayK8644isanopenerofallL-typechannels(CaV1family).Inthepresenceofthisagonist,channelstendtoopenforlongerperiodscausingalargeincreaseinthechannelmacroscopicresponse.
Thenetactivityoftheracemicmixisthatofthe(-)-enantiomeragonisticactivity.
(±)-BayK8644isawidelyusedL-typeCa2+channelactivatorinvariousneuronal,muscle,endocrine,thyroidandothercelltypes.1-3
TargetL-typeCa2+ channels
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Lastupdate:24/09/2019
(±)-BayK8644(#B-350)isahighlypure,synthetic,andbiologicallyactivecompound.
Forresearchpurposesonly,notforhumanuse
Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。
