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蚂蚁淘在线 / 品牌中心 / Discovery / Discovery Antibodies/Anti-KCNN2 (KCa2.2, SK2) Antibody/50 µl/APC-028-50 µl

Discovery Antibodies/Anti-KCNN2 (KCa2.2, SK2) Antibody/50 µl/APC-028-50 µl

价格
￥7340.00
货号：APC-028-50µl
浏览量：127
品牌：Discovery
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商品描述
Cat#:APC-028
AlternativeNameSKCa2, Smallconductancecalcium-activatedpotassiumchannelprotein2, Apamin-sensitivesmallconductanceCa2+-dependentK+channel
  • LyophilizedPowder
  • AntigenIncl.
    • Type:Polyclonal
    Host:Rabbit
    Reactivity:h,m,r
      • Peptide(C)ETQMENYDKHVTYNAERS,correspondingtoaminoacidresidues542-559ofratKCNN2 (Accession P70604).Intracellular,C-terminalpart.
        Anti-KCNN2 (KCa2.2, SK2) Antibody
    Accession(Uniprot)NumberP70604
    GeneID54262
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyMouse-17/18aminoacidresiduesidentical;human-16/18aminoacidresiduesidentical;chicken-14/18aminoacidresiduesidentical.
    RRIDAB_2040126.
    PurityAffinitypurifiedonimmobilizedantigen.
    FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    IsotypeRabbitIgG.
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution25µl,50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
    Antibodyconcentrationafterreconstitution0.8mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
    NegativecontrolantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
    Negativecontrolantigenreconstitution100µldoubledistilledwater(DDW).
    Negativecontrolantigenstorageafterreconstitution-20°C.
    PreadsorptionControl1μgpeptideper1μgantibody.
    StandardqualitycontrolofeachlotWesternblotanalysis.
    Applications:ic,if,ih,ip,wb
    Mayalsoworkin:ifc*
      • Anti-KCNN2 (KCa2.2, SK2) Antibody
        Westernblotanalysis ofratbrainmembranes:
        1. Anti-KCNN2(KCa2.2,SK2) Antibody#APC-028),(1:200).
        2.Anti-KCNN2(KCa2.2,SK2)Antibody,preincubatedwiththenegativecontrolantigen.
        HumanJurkatTcells(1.5µg/ml)(Desai,R. etal. (2000) J.Biol.Chem. 275,39954.).

        Humanandmouseheart(Xu,Y. etal. (2003) J.Biol.Chem. 278, 49085.).
      • Rathippocampalslices(2µg/ml)(Kramar,E.A. etal. (2004) J.Neurosci. 24, 5151.).
      • Anti-KCNN2 (KCa2.2, SK2) Antibody
        ExpressionofKCNN2(SK2)inratcerebralcortex
        ImmunohistochemicalstainingofKCNN2 channelsusing Anti-KCNN2(KCa2.2,SK2) Antibody (#APC-028)inratcerebralcortex.Pictureshowingthethethirdlayer.Notethatbothneural(blackarrows)andglialcells(greenarrows)showintensestaining.ReactionproductisredandcounterstainisH&E.
      • Ratneuronsfromhippocampus(1:200)(Wang,W. etal. (2011) Neuropharmacology 60, 901.).
    References
    • 1.Kohler,M.etal.(1996)Science273,1709.
    • 2.Xia,X.M.etal.(1998)Nature395,503.
    • 3.Stocker,M.(2004)Nat.Rev.Neurosci.5,758.

      • KCa2.2 (KCNN2,SK2)isamemberoftheCa2+-activatedK+ channelfamilywithsmallconductancethatincludes KCa2.1 (KCNN1,SK1)and KCa2.3 (KCNN3,SK3).ThechannelisvoltageinsensitiveandisactivatedbyintracellularCa2+ inthesubmicromolarrange.Ithas,though,asimilartopologytothatofvoltage-dependentK+ channels(KV channels),thatissixtransmembranedomainsandintracellularN-andC-termini.ThefunctionalchannelofalltheKCa2familymembersisamultimericproteincomposedoffourpore-formingsubunits.

        KCa2channelsareextremelysensitivetothelevelsofintracellularCa2+ andconcentrationsaslowas300-700nMcanopenthechannelsveryrapidly(5-15ms).Hence,theKCa2channelsarehighlysensitiveandfastCa2+ sensorsresemblingotherknownCa2+-bindingproteins.ThistypeofCa2+-dependentactivationisachievedbytheconstitutivebindingoftheKCa2channelstocalmodulin,ahighlyexpressedCa2+-bindingproteinviaacalmodulin-bindingdomainsituatedatthecytoplasmicC-terminus.

        Pharmacologically,theKCa2channelsaretheonlyknowntargetsofthebeevenomtoxin Apamin,withKCa2.1beingtheleastsensitive,KCa2.2themostsensitiveandKCa2.3showingintermediatesensitivity.

        KCa2.2ispredominantlyexpressedinthenervoussystemalthoughexpressioninendothelialcells,heartandliverhasbeendescribed.

        KCa2.2isknowntobeinvolvedintheregulationofneuronalexcitABIlity.TheydosomainlyviaaphenomenonknownasafterhyperpolarizationinwhichKCa2channelsopeninresponsetoincreasedintracellularCa2+ concentrationsthatresultfromtheentryofextracellularCa2+ throughvoltage-dependentCa2+ channelsduringactionpotentials.Inthisway,KCa2channelseffectivelyformaCa2+-mediatedfeedbackloop.

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IF-Immunofluorescence,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    LyophilizedPowderNegativeControlAntigenIncluded
    receivea25µlfreetrialsample!
    Lastupdate:08/11/2019

    Anti-KCNN2(KCa2.2,SK2)Antibody(#APC-028)isahighlyspecificantibodydirectedagainstanepitopeoftheratprotein.Theantibodycanbeusedinwesternblot,immunocytochemistry,immunohistochemistry,andimmunoprecipitationapplications.IthasbeendesignedtorecognizeKCNN2fromhuman,rat,andmousesamples.

    Forresearchpurposesonly,notforhumanuse
    Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。

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