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蚂蚁淘在线 / 品牌中心 / Discovery / Discovery Antibodies/Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody/5 x 50 µl/AER-051-F-5 x 50 µl

Discovery Antibodies/Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody/5 x 50 µl/AER-051-F-5 x 50 µl

价格

￥25860.00

货号：AER-051-F-5x50µl

浏览量：127

品牌：Discovery

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商品描述

Cat#:AER-051-F

AlternativeNameEMR1hormonereceptor, EGF-likemodule-containingmucin-likehormonereceptor-like1,AdhesionGprotein-coupledreceptorE1,TM7LN3,F4/80,GPF480

LyophilizedPowder

Type:Polyclonal

Host:Rabbit

Reactivity:h,m,r

Immunogen-+

Accession(Uniprot)NumberQ14246

GeneID2015

PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.

HomologyMouse,Rat–11/14aminoacidresiduesidentical.

RRIDAB_2756589.

PurityAffinitypurifiedonimmobilizedantigen.

FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN₃.

IsotypeRabbitIgG.

LabelFluoresceinisothiocyanate(FITC).

StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.

Reconstitution50µldoubledistilledwater(DDW).

Antibodyconcentrationafterreconstitution1mg/ml.

StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).

StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#AER-051),anddirectflowcytometry(labeledantibody).

Applications:fc

Mayalsoworkin:ic*,lci*

Directflowcytometry-+

References

1.Legrand,F.etal.(2014)J.AllergyClin.Immunol.133,1439.

2.McKnight,A.J.andGordon,S.(1998)J.Leukoc.Biol.63,271.

3.Araç,D.etal.(2012)EMBOJ.31,1364.

Scientificbackground-+

LyophilizedPowder

Lastupdate:16/09/2019

Anti-EMR1(ADGRE1)(extracellular)Antibody (#AER-051)isahighlyspecificantibodydirectedagainstanepitopeofthehumanprotein.Theantibodycanbeusedinwesternblotandlivecellimagingapplications.Itrecognizesanextracellularepitopeandisthusidealfordetectingthereceptorinlivingcells.IthasbeendesignedtorecognizeEMR1fromhuman,mouse,andratsamples.

Anti-EMR1(ADGRE1)(extracellular)–FITCAntibody (#AER-051-F)is directlyconjugatedtofluoresceinisothiocyanate(FITC). The antibodycanbeusedinimmunofluorescentapplicationssuchasdirectlivecellflowcytometry.

Forresearchpurposesonly,notforhumanuse

Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。

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Discovery Antibodies/Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody/5 x 50 µl/AER-051-F-5 x 50 µl

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