Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Negative control antigen reconstitution 100 µl double distilled water (DDW).
Negative control antigen storage after reconstitution -20°C.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard quality control of each lot Western blot analysis.
Applications: ic, if, lci, wb
May also work in: ifc*, ih*, ip*
Western blot analysis of rat fat (lanes 1 and 6), mouse fat (lanes 2 and 7), human Burkitt's lymphoma (Raji) (lanes 3 and 8), rat basophilic leukemia (RBL) (lanes 4 and 9) and human promyelocytic leukemia (HL-60) (lanes 5 and 10) lysates:
1-5. Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051), (1:400). 6-10. Anti-EMR1 (ADGRE1) (extracellular) Antibody, preincubated with the negative control antigen.
Expression of EMR1 in rat RBL cells
Cell surface detection of EMR1 in intact living rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells using Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red), (1:100). B. Live view of the cells. C. Merged image of panels A and B.
References
1. Legrand, F. et al. (2014) J. Allergy Clin. Immunol. 133, 1439.
2. McKnight, A.J. and Gordon, S. (1998) J. Leukoc. Biol. 63, 271.
3. Araç, D. et al. (2012) EMBO J. 31, 1364.
Human epidermal growth factor (EGF)–like module containing mucin-like hormone receptor 1 (EMR1) is a surface receptor with unknown function that belongs to the adhesion family of the G protein-coupled receptors (GPCR). This receptor is encoded by the ADGRE1 and like all GPCRs, contains seven transmembrane domains, an intracellular C-terminus and an extracellular N-terminus. The NH2 domain has six EGF-like domains.
Adhesion GPCRs share a long N-terminal extracellular sequences comprising multiple domains, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties1,2.
EMR1 protein is a homolog of F4/80, a murine protein that is widely being used as a marker of murine macrophage populations1.
Human EMR1 is exclusively expressed on mature eosinophils in the blood and bone marrow and in nasal polyps. Thus, EMR1 is considered to be a potential therapeutic target for the treatment of eosinophilic disorders1,3.
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry,IE- Indirect ELISA, IFC- Indirect flow cytometry, IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation,LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Lyophilized PowderNegative Control Antigen Included
Click here to receivea 25 µl free trial sample!
Certificate of Analysis MSDS
Last update: 10/11/2019
Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell imaging applications. It recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize EMR1 from human, mouse, and rat samples.
For research purposes only, not for human use
Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。
Lyophilized Powder 
Antigen Incl. 
Western blot analysis of rat fat (lanes 1 and 6), mouse fat (lanes 2 and 7), human Burkitt's lymphoma (Raji) (lanes 3 and 8), rat basophilic leukemia (RBL) (lanes 4 and 9) and human promyelocytic leukemia (HL-60) (lanes 5 and 10) lysates:1-5. Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051), (1:400).
Expression of EMR1 in rat RBL cellsCell surface detection of EMR1 in intact living rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells using Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red), (1:100). B. Live view of the cells. C. Merged image of panels A and B.
