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商品描述
Alternative Name MT3, MT-3
Lyophilized Powder Lyophilized Powder
This product is freeze dried. All water molecules have been removed.
Bioassay Tested Bioassay Tested
Every lot is tried & tested in a relevant biological assay.
Our Bioassay
Origin Natural protein isolated from Dendroaspis angusticeps (Eastern green mamba) (Naja angusticeps).
MW: 7379 Da.
Purity: >99% (HPLC)
Form Lyophilized powder.
Effective concentration 100-1000 nM.
Sequence LTCVTKNTIFGITTENCPAGQNLCFKRWHYVIPRYTEITRGCAATCPIPENYDSIHCCKTDKCNE.
Modifications Disulfide bonds between Cys3-Cys24, Cys17-Cys42, Cys46-Cys57and Cys58-Cys63.
Molecular formula C319H489N89O97S8.
Activity Muscarinic receptor 4 and adrenoceptor antagonist1-4.
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- Jolkkonen, M. et al. (1994) FEBS Lett. 352, 91.
- Karlsson, E. et al. (1994) Ann. N.Y. Acad. Sci. 710, 153.
- Caulfield, M.P. and Birdsall, N.J. (1998) Pharm. Rev. 50, 279.
- Nareoja, K. et al. (2011) Br. J. Pharmacol. 164, 538.
Accession number P81031.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions Up to two weeks at 4°C or three months at -20°C.
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Alomone Labs Muscarinic Toxin 3 inhibits Muscarinic Receptor 4 expressed in CHO-K1 cells.CHO-K1 cells were co-transfected with human M4 and Gα15 in order to couple the M4 receptor to the Ca2+ signaling pathway. Ca2+ concentrations were monitored using FLIPR. Cells were first incubated in the presence of different concentrations of Muscarinic Toxin 3 (#M-140) followed by Oxotremorine. The percentage of M4 receptor inhibition was plotted versus different concentrations of Muscarinic Toxin 3. The IC50 value of Muscarinic Toxin 3 was between 100 to 1000 nM.
References - Scientific background
- 1. Bradley, K.N. (2000) Pharmacol. Ther. 85, 87.
- 2. Nareoja, K. et al. (2011) Br. J. Pharmacol. 164, 538.
- Muscarinic toxins are isolated from the venom of the mamba species and are composed of 65 to 66 amino acid residues with four intramolecular disulfide bridges. One of these is muscarinic toxin 3 (MT-3) which was isolated from the green mamba (Dendroaspis augusticeps) and is composed of 65 amino acid residues1. A recent study investigated the interaction of MT3 with cloned receptors (mAChRs and adrenoceptors) expressed in insect cells by radioligand binding. MT3 appears to have a broad spectrum of targets showing high-affinity binding (IC50 = 1-10 nM) to M4 mAChR, α1A-, α1D- and α2A-adrenoceptors and lower affinity binding (IC50 > 25 nM) to α1B- and α2C-adrenoceptors and M1 mAChR2.
Target M1, M4 muscarinic receptors, adrenoceptors
Net Peptide Content: 100%
Lyophilized Powder
Click here to receive a 50 µg free trial sample!
Last update: 04/11/2019
Muscarinic Toxin 3 (#M-140) is a highly pure, natural, and biologically active peptide toxin.
For research purposes only, not for human use
Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。
