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蚂蚁淘在线 / 品牌中心 / Discovery / Discovery Antibodies/Guinea pig Anti-CaV1.2 (CACNA1C) Antibody/0.2 ml/AGP-001-0.2 ml

Discovery Antibodies/Guinea pig Anti-CaV1.2 (CACNA1C) Antibody/0.2 ml/AGP-001-0.2 ml

价格
￥9500.00
货号：AGP-001-0.2ml
浏览量：127
品牌：Discovery
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商品描述
Cat#:AGP-001
AlternativeNameVoltage-dependentL-typecalcium channelsubunitα1C
  • LyophilizedPowder
  • AntigenIncl.
    • Type:Polyclonal
    Host:Guineapig
    Reactivity:h,m,r
      • Peptide(C)TTKINMDDLQPSENEDKS,correspondingtoaminoacidresidues848-865ofratCaV1.2(Accession P22002).IntracellularloopbetweendomainsIIandIII.
        Guinea pig Anti-CaV1.2 (CACNA1C) Antibody
    Accession(Uniprot)NumberP22002
    GeneID24239
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyMouse-identical;guineapig-17/18aminoacidresiduesidentical;human,rabbit-16/18aminoacidresiduesidentical.
    RRIDAB_11219156.
    PurityAffinitypurifiedonimmobilizedantigen.
    FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    IsotypeGuineapigtotalIgG.
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
    Antibodyconcentrationafterreconstitution0.8mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
    NegativecontrolantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
    Negativecontrolantigenreconstitution100µldoubledistilledwater(DDW).
    Negativecontrolantigenstorageafterreconstitution-20°C.
    PreadsorptionControl1µgpeptideper1µgantibody.
    StandardqualitycontrolofeachlotWesternblotanalysis.
    Applications:if,ih,wb
    Mayalsoworkin:ic*,ifc*,ip*
      • Guinea pig Anti-CaV1.2 (CACNA1C) Antibody
        Westernblotanalysisofratbrainmembrane:
        1. Guineapig Anti-CaV1.2 (CACNA1C) Antibody(#AGP-001),(1:200).
        2.GuineapigAnti-CaV1.2(CACNA1C)Antibody,preincubatedwiththenegativecontrolantigen.
        Guinea pig Anti-CaV1.2 (CACNA1C) Antibody
        WesternblotanalysisofCaV1.2-transfected Xenopus oocytes(lane1)andnon-transfectedoocyteslysates(lane2):
        1. Guineapig Anti-CaV1.2 (CACNA1C) Antibody(#AGP-001),(1:200)in CaV1.2(CACNA1C)ChannelOverexpressedMembraneFractions(LX-104).
        2.GuineapigAnti-CaV1.2(CACNA1C)Antibodyinnon-transfectedoocytes.
      • Guinea pig Anti-CaV1.2 (CACNA1C) Antibody
        Immuno-colocalizationofCaV1.2andGABA(A) α1Receptorinratcerebellum
        ImmunohistochemicalstainingofratcerebellumusingGuineapig Anti-CaV1.2 (CACNA1C) Antibody(#AGP-001)and Anti-GABA(A) α1Receptor(extracellular)-ATTO-488Antibody (#AGA-001-AG). A.CaV1.2(red)isdetectedmostlyinPurkinjecells(arrow).B.Inthesamesection, GABA(A)α1Receptor(green)isobservedinthegranulelayer.C.MergeofthetwoimagessuggestssomecolocalizationbetweenCaV1.2andGABA(A)α1ReceptorintheratgranulelayerbutonlyCaV1.2appearsinPurkinjecells.
        Guinea pig Anti-CaV1.2 (CACNA1C) Antibody
        ExpressionofCaV1.2inhumanatria
        ImmunohistochemicalstainingofhumanleftatriumusingGuineapigAnti-CaV1.2 (CACNA1C) Antibody(#AGP-001),(1:100).
        ThepicturewaskindlyprovidedbyDr.VanWagoner,D.R.fromtheDepartmentofMolecularCardiology,ClevelandClinic,Cleveland,Ohio,USA.Lovano,B.andPeterson,J.collectedthedata.
        Guinea pig Anti-CaV1.2 (CACNA1C) Antibody
        ExpressionofCaV1.2inratheart
        ImmunohistochemicalstainingofratheartparaffinembeddedsectionsusingGuineapig Anti-CaV1.2 (CACNA1C) Antibody(#AGP-001).A.CaV1.2staining(green)appearsmainlyinthecardiacmuscle,andinalesserintensityinthetunicaintimalayerofthesmoothmuscleofthemusculararteries.B.NuclearstainingusingDAPIasthecounterstain.C.MergedimagesofAandB.
        Guinea pig Anti-CaV1.2 (CACNA1C) Antibody
        ExpressionofCaV1.2inmousehippocampus
        Immunohistochemicalstainingofmousedentategyrususing Guineapig Anti-CaV1.2 (CACNA1C) Antibody(#AGP-001).A.CaV1.2(green)appearedintheoutermolecularlayerofthedentategyrusandinthegranulelayer.B.CounterstainwithDAPI(blue)outlinesthegranulelayerofthedentategyrus.
    References
    • 1.Bauer,C.S.etal.(2010)Curr.Opin.Neurobiol.20,563.
    • 2.Arikkath,J.etal.(2003)Curr.Opin.Neurobiol.13,298.
    • 3.Catterall,W.A.(2000)Annu.Rev.Cell.Dev.Biol.16,521.
    • 4.Davies,A.etal.(2007)TrendsPharmacol.Sci.28,220.
    • 5.Dai,S.etal.(2009)Physiol.Rev.89,411.
    • 6.Zuccotti,A.etal.(2011)TrendsPharmacol.Sci.32,366.
    • 7.Hell,J.W.etal.(1996)Proc.Natl.Acad.Sci.U.S.A.93,3362.
    • 8.DeJongh,K.S.etal.(1996)Biochemistry35,10392.
    • 9.Gao,T.etal.(2001)J.Biol.Chem.276,21089.
    • 10.Liao,P.etal.(2010)Pflugers.Arch.460,353.

      • Voltage-gatedCa2+ channels(CaV),enablethepassageofCa2+ ionsinavoltagedependentmanner.Theseheteromericentitiesareformedinpartbythepore-forming α1subunitwhichdeterminesthebiophysicalandpharmacologicalpropertiesofthechannel1.

        L-typeCa2+ channelsmakeuponeofthreevoltage-gatedCa2+ channelfamilies.Fourdifferent α1isoforms(CaV1.1toCaV1.4)belongtotheL-typesubfamily.Structurally,each α1subunithasfourhomologousdomains(I-IV)andeachdomainhasasixtransmembranesection.Likemanyothervoltage-gatedchannels,L-typeCa2+ channelshaveauxiliarysubunitswhichareresponsIBLeformodulatingthesurfaceexpressionandpropertiesofthechannels2-5.

        CaV1.1ismostlyexpressedintheskeletalmuscle,whileCaV1.4ismainlydetectedintheretina.TheexpressionofbothCaV1.2and CaV1.3 ismoreextensiveandincludesneurons,heart,smoothmuscle,innerear,retinaandpancreas6.L-typeCa2+ channelsareinvolvedinandmodulateavarietyofphysiologicalfunctionssuchasmusclecontraction,hormonesecretion,neuronalexcitABIlityandgeneexpression5.

        CaV1.2undergoesvariouspost-translationalmodifications.Forexample,itcanundergoproteolyticcleavageatitsC-terminal.Thiscleavagehasbeenshowntotakeplaceinneuronsfollowingtheactivationof NMDA receptors5,7 andintheheart5,8,9.Thecleavedmoietycanstillinteractwiththechannelanditsgeneralpurposeistomodulatechannelactivity5.OtherpostranslationmodificationsofthechannelincludephosphorylationofCaV1.2byanumberofkinasessuchasPKA,PKC,SrcandCaMKII5.Inaddition,itisnotsurprisingthatphosphatasesalsoregulatechannelactivity,astheyarerequiredtoantagonizetheactivityofthevariouskinasesknowntophosphorylateCaV1.2 5.

        ThefactthatCaV1.2playsaprominentroleinpropercardiacfunctionhaspromptedendlessstudiesregardingitsregulation.Suchstudieshaveconcludedthatdysregulationofthechannelleadstoanomaliesinheartcontractionandthusheartfailure5.Likewise,CaV1.2defectshavebeendetectedinautismandbipolardisorder10.

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IF-Immunofluorescence,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    LyophilizedPowderNegativeControlAntigenIncluded
    receivea25µlfreetrialsample!
    Image&Title:

    Guinea pig Anti-Cav1.2 (CACNA1C) Antibody
    Immuno-colocalizationofCaV1.2andGABA(A) α1Receptorinratandmousehippocampus    ImmunohistochemicalstainingofmouseandrathippocampaldentategyrususingGuineapig Anti-CaV1.2 (CACNA1C) Antibody(#AGP-001)and Anti-GABA(A)α1Receptor(extracellular)-ATTO-488Antibody (#AGA-001-AG)inthesamesection.BothCaV1.2(red)andGABA(A)α1Receptor(green)aredetectedinneuron-shapedcells(arrows).StainingsuggestspartialcolocalizationbetweenCaV1.2andGABA(A)α1Receptorinasub-populationofdentategyrusneurons.A.Mousehippocampus.B.Rathippocampus.

    Lastupdate:21/11/2019

    GuineapigAnti-CaV1.2(CACNA1C)Antibody(#AGP-001),raisedinguineapigs,isahighlyspecificantibodydirectedagainstanepitopeoftheratprotein.Theantibodycanbeusedinwesternblotandimmunohistochemistryapplications.ItandhasbeendesignedtorecognizeCaV1.2frommouse,ratandhumansamples.TheantigenusedtoimmunizeguineapigsisthesameasAnti-CaV1.2(CACNA1C)Antibody(#ACC-003)raisedinrabbit.Ourlineofguineapigantibodiesenablesmoreflexibilitywithourproductssuchasimmuno-colocalizationstudies,immunoprecipitation,etc.

    Forresearchpurposesonly,notforhumanuse
    Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。

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