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蚂蚁淘在线 / 品牌中心 / Discovery / Discovery Antibodies/Guinea pig Anti-CaV1.2 (CACNA1C) Antibody/50 µl/AGP-001-50 µl
商品描述
AlternativeNameVoltage-dependentL-typecalcium channelsubunitα1C
KOValidatedKOValidated
Thespecificityofthisantibodyhasbeenvalidatedinaknockout(KO)orknockdown(KD)system.
LyophilizedPowderLyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl.AntigenIncl.
ThisantibodyisshippedwithitsantigenFREEofcharge!
Type:Polyclonal
Host:Rabbit
Reactivity:h,m,r
- Peptide(C)TTKINMDDLQPSENEDKS,correspondingtoaminoacid residues848-865ofratCaV1.2(Accession P22002).IntracellularloopbetweendomainsIIandIII.
Accession(Uniprot)NumberP22002
GeneID24239
PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
HomologyRat,mouse -8/11 aminoacidresiduesidentical.
RRIDAB_2039771.
PurityAffinitypurifiedonimmobilizedantigen.
FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
IsotypeRabbitIgG.
StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
Antibodyconcentrationafterreconstitution0.8mg/ml.
StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
NegativecontrolantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
Negativecontrolantigenreconstitution100µldoubledistilledwater(DDW).
Negativecontrolantigenstorageafterreconstitution-20°C.
PreadsorptionControl1µgpeptideper1µgantibody.
StandardqualitycontrolofeachlotWesternblotanalysis.
Applications:ic,if,ifc,ih,ip,wb
Westernblotanalysisofratbrainmembranes:1. Anti-CaV1.2(CACNA1C)Antibody(#ACC-003),(1:200).
2.Anti-CaV1.2(CACNA1C)Antibody,preincubatedwiththecontrolpeptideantigen.
WesternblotanalysisofCaV1.2-transfected Xenopus oocytes(lane1)andnon-transfectedoocyteslysates(lane2):1. Anti-CaV1.2 (CACNA1C) Antibody(#ACC-003),(1:200)in CaV1.2(CACNA1C)ChannelOverexpressedMembraneFractions(LX-104).
2.Anti-CaV1.2(CACNA1C)Antibodyinnon-transfectedoocytes.Humancardiactissue(Crossman,D.J. etal. (2011) PLoSONE 6, e17901.).
- CaV1.2transfectedHEK-293cellsandmouseheartlysate(Rougier,J.S. etal. (2011) J.Biol.Chem. 286, 8829.).
ExpressionofCaV1.2inmousecerebellumImmunohistochemicalstainingofmousecerebellumwith Anti-CaV1.2 (CACNA1C) Antibody(#ACC-003).A.CaV1.2(red)appearsinPurkinjecells(horizontalarrows)andisdistributeddiffuselyinthemolecularlayer(Mol)includinginPurkinjedendrites(verticalarrows).B.StainingofPurkinjenervecellswithmouseanti-Calbindin28K (green)demonstratesthelocationofdendritesinthemolecularlayer.C.MergedimageofpanelsAandB.Mouseatrioles(1:300)(Howitt,L. etal. (2013) J.Physiol. 591, 2157.).
- Ratinsulinomacells(RIN)(Parkash,J.(2011) LifeSci. 88, 257.).Mousechromaffincells(1:200).(Perez-Alvarez,A. etal. (2011) J.NeuRochem. 116, 105.).
- Ratmastcells(RBL-2H3cells,1μg/5x105 cells).(Yoshimaru,T. etal. (2009) Mol.Immunol. 46, 1267.).Thecontrolantigenisnotsuitableforthisapplication.
References
- 1.Catterall,W.A.etal.(2003)Pharmacol.Rev.55,579.
- 2.IUPHAR
- 3.Hu,X.Q.etal.(1998)J.Biol.Chem.273,5337.
- 4.Kreuzberg,U.etal.(2000)AmJ.Physiol.278,H723.
- 5.Allard,B.etal.(2000)J.Biol.Chem.275.25556.
AllL-typecalciumchannelsareencodedbyoneoftheCaV1channelgenes.ThesechannelsplayamajorroleasaCa2+ entrypathwayinskeletal,cardiacandsmoothmusclesaswellasinneurons,endocrinecellsandpossIBLyinnon-excitablecellssuchashematopoeticandepithelialcells.AllCaV1channelsareinfluencedbydihydropyridines(DHP)andarealsoreferredtoasDHPreceptors.
WhiletheCaV1.1andCaV1.4isoformsareexpressedinrestrictedtissues(skeletalmuscleandretina,respectively),theexpressionofCaV1.2isubiquitousand CaV1.3 channelsarefoundintheheart,brainandpancreas.SeveralpeptidyltoxinsaredescribedthatarespecificL-typechannelblockers,butsofarnoselectiveblockerforoneoftheCaV1isoformshavebeendescribed.TheseincludetheMambatoxins Calcicludine (#SPC-650), Calciseptine (#C-500)and FS-2 (#F-700).
LyophilizedPowderNegativeControlAntigenIncluded
Image&Title:
ExpressionofCaV1.2inratcardiomyocytes.ImmunocytochemicalstainingofadultratcardiomyocytesusingAnti-CaV1.2(CACNA1C) Antibody(#ACC-003).CaV1.2staining(green)isobservedinaregularlyspacedarray,aswellassurfacesarcolemmalstaining.β-arrestinstainingisinred.AdaptedfromHermosilla,T.etal.(2017)Sci.Rep.7,10131.withpermissionofSPRINGERNATURE.
ExpressionofCaV1.2inratcardiomyocytes.ImmunocytochemicalstainingofadultratcardiomyocytesusingAnti-CaV1.2(CACNA1C) Antibody(#ACC-003).CaV1.2staining(green)isobservedinaregularlyspacedarray,aswellassurfacesarcolemmalstaining.β-arrestinstainingisinred.AdaptedfromHermosilla,T.etal.(2017)Sci.Rep.7,10131.withpermissionofSPRINGERNATURE.
Lastupdate:21/11/2019
Anti-CaV1.2(CACNA1C)Antibody(#ACC-003)isahighlyspecificantibodydirectedagainstanepitopeoftheratprotein.Theantibodycanbeusedinwesternblot,immunoprecipitation,immunohistochemistry,immunocytochemistry,andindirectflowcytometryapplications.IthasbeendesignedtorecognizeCaV1.2frommouse,rat,andhumansamples.
Forresearchpurposesonly,notforhumanuse
Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。
