AlternativeName Calciumhomeostasismodulator1,FAM26C
LyophilizedPowder LyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl. AntigenIncl.
ThisantibodyisshippedwithitsantigenFREEofcharge!
Type: Polyclonal
Host: Rabbit
Reactivity: h,m,r
Immunogen- + Peptide(C)EAMNHDLELGHTHG,correspondingtoaminoacidresidues252-265ofhumanCALHM1(Accession Q8IU99).Intracellular,C-terminus.
Accession(Uniprot)Number Q8IU99
GeneID 255022
Peptideconfirmation Confirmedbyaminoacidanalysisandmassspectrometry.
Homology Rat,mouse-identical.
RRID AB_11125728.
Purity Affinitypurifiedonimmobilizedantigen.
Form Lyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3 .
Isotype RabbitIgG.
Storagebeforereconstitution Theantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
Reconstitution 25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
Antibodyconcentrationafterreconstitution 0.8mg/ml.
Storageafterreconstitution Thereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
Negativecontrolantigenstoragebeforereconstitution Lyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
Negativecontrolantigenreconstitution 100µldoubledistilledwater(DDW).
Negativecontrolantigenstorageafterreconstitution -20°C.
PreadsorptionControl 1µgpeptideper1µgantibody.
Standardqualitycontrolofeachlot Westernblotanalysis.
Applications: if,ih,wb
Mayalsoworkin: ic* ,ifc* ,ip*
Westernblot- + Westernblotanalysisofratbrain(lanes1and4),mousebrain(lanes2and5),U-87MG(lanes3and6)andC6(lanes4and8)lysates:
1.Anti-CALHM1Antibody (#ACC-101),(1:200).
Immunohistochemistry- + Expressionof CALHM1inrathippocampus
ImmunohistochemicalstainingofrathippocampususingAnti-CALHM1Antibody (#ACC-101).A.Stainingof CALHM1appearsonlyinthehippocampalCA1pyramidallayer(red).B.NuclearstainingusingDAPIasthecounterstain(blue).C.MergeofAandB.
References
1.Green,K.N.andLaFerla,F.M.(2008)Neuron 59, 190. 2.Koppel,J.etal. (2011)Mol.Med. 17, 974. 3.Dreses-Werringloer,U.etal. (2008)Cell 133, 1149. Scientificbackground- + AmisbalanceinCa2+ homeostasisseemstoberelatedtothedevelopmentofAlzheimer’sdisease(AD).Indeed,neuronalCa2+ balancemayaffectthelevelsofproteinsassociatedwithAD,suchasamyloid-beta(Ab)andtau1 .
Calciumhomeostasismodulator1(CALHM1)isacerebralCa2+ channelcomponentresponsIBL 2+ levelsandAbmetabolism2,3 .CALHM1isathreetransmembraneglycoprotein,mostlylocalizedtotheendoplasmicreticulumalthoughitcanbedetectedattheplasmamembrane.AfunctionalchannelisformedbymultiplesubunitswhichhassomestructuralsimilaritiestotheNMDAreceptor.Itisexpressedinallbrainregions1 .
Inculturedcells,overexpressionofCALHM1increasesintracellularCa2+ levelsandreducesAbaccumulation,therebyreinforcingitsimplicationinthedevelopmentofAD.Indeed,apolymorphisminthegenewasfoundtoaffecttheonsetofAD2 .
LyophilizedPowder NegativeControlAntigenIncluded
Clickhereto receivea50µl freetrialsample!
Lastupdate:10/11/2019
Anti-CALHM1Antibody (#ACC-101)isahighlyspecificantibodydirectedagainstanepitopeofthehumancalciumhomeostasismodulatorprotein1.Theantibodycanbeusedinwesternblotandimmunohistochemistryapplications.IthasbeendesignedtorecognizeCALHM1fromrat,mouseandhumansamples.
Forresearchpurposesonly,notforhumanuse
Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。
LyophilizedPowder
AntigenIncl.
Westernblotanalysisofratbrain(lanes1and4),mousebrain(lanes2and5),U-87MG(lanes3and6)andC6(lanes4and8)lysates:1.Anti-CALHM1Antibody (#ACC-101),(1:200).
Expressionof CALHM1inrathippocampusImmunohistochemicalstainingofrathippocampususingAnti-CALHM1Antibody (#ACC-101).A.Stainingof CALHM1appearsonlyinthehippocampalCA1pyramidallayer(red).B.NuclearstainingusingDAPIasthecounterstain(blue).C.MergeofAandB.
