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蚂蚁淘在线 / 品牌中心 / Discovery / Discovery Antibodies/Guinea pig Anti-GluR1 (GluA1) (extracellular) Antibody/0.2 ml/AGP-009-0.2 ml

Discovery Antibodies/Guinea pig Anti-GluR1 (GluA1) (extracellular) Antibody/0.2 ml/AGP-009-0.2 ml

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￥9500.00
货号：AGP-009-0.2ml
浏览量：127
品牌：Discovery
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商品描述
Cat#:AGP-009
AlternativeNameAMPAReceptor1,Glutamatereceptor1,Ionotropicglutamatereceptor1,AMPA-selectiveglutamatereceptor1,GRIA1, GluR-A, GluR-K1
  • LyophilizedPowder
  • AntigenIncl.
    • Type:Polyclonal
    Host:Guineapig
    Reactivity:h,m,r
      • PeptideRTSDSRDHTRVDWKR(C),correspondingtoaminoacidresidues271-285ofratGluR1(Accession P19490).Extracellular,N-terminus.
        Guinea pig Anti-GluR1 (GluA1) (extracellular) Antibody
    Accession(Uniprot)NumberP19490
    GeneID50592
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyMouse-identical;human,pig,dog-13/14aminoacidresiduesidentical.
    RRIDAB_2340961.
    PurityAffinitypurifiedonimmobilizedantigen.
    FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    IsotypeGuineapigtotalIgG.
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
    Antibodyconcentrationafterreconstitution0.8mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
    NegativecontrolantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
    Negativecontrolantigenreconstitution100µldoubledistilledwater(DDW).
    Negativecontrolantigenstorageafterreconstitution-20°C.
    PreadsorptionControl1µgpeptideper1µgantibody.
    StandardqualitycontrolofeachlotWesternblotanalysis.
    Applications:ic,if,ih,lci,wb
    Mayalsoworkin:ifc*,ip*
      • Guinea pig Anti-GluR1 (GluA1) (extracellular) Antibody
        Westernblotanalysis ofrat(lanes1and3)andmouse(lanes2and4)brainlysates:
        1,2.Guineapig Anti-GluR1(GluA1)(extracellular) Antibody(#AGP-009), (1:200).
        3,4.GuineapigAnti-GluR1(GluA1)(extracellular)Antibody,preincubatedwiththenegativecontrolantigen.
      • Guinea pig Anti-GluR1 (GluA1) (extracellular) Antibody
        ExpressionofGluR1inrathippocampus
        Immunohistochemicalstainingofperfusion-fixedfrozenratbrainsections usingGuineapigAnti-GluR1(GluA1)(extracellular)Antibody (#AGP-009),(1:400),followedbyanti-rabbit-Cy2antibody(green).GluR1stainingappearsinneuronaloutlines(horizontalarrows)andintheinnermolecularlayerofthedentategyrus(verticalarrow).NucleiarestainedwithDAPI(blue).
      • Guinea pig Anti-GluR1 (GluA1) (extracellular) Antibody
        Immuno-colocalizationofGluR1andVesicularGABATransporterinhumanU-87MGcells
        Cellsurfacedetectionof GluR1andVesicularGABATransporterinhumanglioblastomaU-87MG.ExtracellularstainingofliveintactcellswithGuineapigAnti-GluR1(GluA1)(extracellular)Antibody (#AGP-009),(1:25),followedbygoatanti-guineapig-AlexaFluor-488secondaryantibody(green).Cellsweresubsequentlyfixed,permeABIlizedandlabeledwith Anti-VesicularGABATransporter(VGAT)Antibody(#AGT-005),(1:200),followedbygoatanti-rabbit-AlexaFluor-594secondaryantibody(red).RepresentativemergedimagesofthedoublelabeledcellsareshowninAandB.
    References
    • 1.Dingledine,R.etal.(1999)Pharmacol.Rev.51,7.
    • 2.Sheng,M.etal.(2001)Cell.105,825.
    • 3.Song,I.etal.(2002)Trends.Neurosci.25,578.

      • AMPAreceptors aremembersofthe glutamatereceptorfamily ofionchannelsthatalsoincludetheNMDAand Kainate receptors.ThethreesubfamiliesarenamedaftertheoriginalsyntheticagoNISTsthatwereidentifiedasselectiveligandsofeachfamily.

        Theα-amino-3-hydroxy-5-methyl-4-isoazolepropionicacid(AMPA)receptorsubfamilyincludesfourmembersAMPA1-AMPA4thatarealsoknownasGluR1-GluR4respectively.

        ThefunctionalAMPAchannelisbelievedtobeatetramer,withmostneuronalAMPAreceptorsbeingactuallyheterotetramerscomposedofAMPA1plus AMPA2 orAMPA2plus AMPA3,althoughhomotetramerscanalsobefound.

        AMPAreceptorsarepermeabletocationsNa+,K+ andCa2+.TheCa2+ permeabilityisdependentonthepresenceofAMPA2:wheneverthissubunitispresent,thechannelwillbeimpermeabletoCa2+.TheCa2+ permeabilityoftheAMPA2subunitisdeterminedbythepresenceofanarginine(R)atacriticalsiteintheporeloopinsteadofaglutamine(Q)presentinthesamesiteintheotherAMPAsubunits.Apost-transcriptionalprocessknownasRNAeditingdeterminesthepresenceofthisR.SincemostAMPA2subunitsintheadultbrainhaveundergoneRNAeditingandmostAMPAreceptorscontaintheAMPA2subunit, mostnativeAMPAreceptorswillbeimpermeabletoCa2+.

        GatingofAMPAreceptorsbyglutamateisextremelyfastandthereforetheAMPAreceptorsmediatemostexcitatory(depolarizing)currentsinthebrainduringbasalneuronalactivity.Thedepolarizationcausedbytheactivationofpost-synapticAMPAreceptorsisnecessaryfortheactivationofNMDAreceptorsthatwillopenonlyinthepresenceofbothglutamateandadepolarizedmembrane.

        Synapticstrength,definedasthelevelofpost-synapticdepolarization,canbelongterm(hencethetermlongtermpotentiation,LTP)andthereforeinducechangesinsignalingandproteinsynthesisintheactivatedneuron.Thesechangesareassociatedwithmemoryformationandlearning.

        ChangesinsynapticstrengtharethoughttoinvolverapidmovementoftheAMPAreceptorsinandoutofthesynapsesandagreatdealofefforthasfocusedinunderstandingthemechanismsthatgovernAMPAreceptortrafficking.

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,
    IF-Immunofluorescence,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    LyophilizedPowderNegativeControlAntigenIncluded
    receivea25µlfreetrialsample!
    Image&Title:Guinea pig Anti-GluR1 (GluA1) (extracellular) Antibody
    ExpressionofGluR1inmouseolfactorybulb.ImmunohistochemicalstainingofmouseolfactorybulbsectionsusingGuineapigAnti-GluR1(GluA1)(extracellular)Antibody (#AGP-009).GluR1staining(green)isdetectedintheglomerularandexternalplexiformlayers(EPL),(rightpanel).GluR1co-localizeswithGFAPinperiglomerularastrocytesandtheirprocessesintheneuropil(mergedpanel).Adaptedfrom Droste,D. etal. (2017) Sci.Rep. 7, 44817.withpermissionofNaturePublishingGroup.
    Lastupdate:11/11/2019

    AlomoneLabsispleasedtoofferanantibodyagainstanextracellularepitopetherationotropicglutamatereceptor1.GuineapigAnti-GluR1(GluA1)(extracellular)Antibody (#AGP-009)raisedinguineapigcanbeusedinwesternblot,immunohistochemistryandimmunocytochemistryapplications.IthasbeendesignedtorecognizeGluR1fromhuman,mouseandratsamples.TheantigenusedtoimmunizeguineapigsisthesameasAnti-GluR1(GluA1)(extracellular)Antibody(#AGC-004)raisedinrabbit.Ourlineofguineapigantibodiesenablesmoreflexibilitywithourproductssuchasimmuno-colocalizationstudies,immunoprecipitation,etc.

    Forresearchpurposesonly,notforhumanuse
    Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。

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