AlternativeName Proto-oncogeneMas,MAS1
LyophilizedPowder LyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl. AntigenIncl.
ThisantibodyisshippedwithitsantigenFREEofcharge!
Type: Polyclonal
Host: GuineaPig
Reactivity: h,m,r
Immunogen- + Peptide(C)KIRKNTWASHSSK,correspondingtoaminoacidresidues212-224oftheratAngiotensin-(1-7)Masreceptor(AccessionP12526).3rd intracellular loop.
Accession(Uniprot)Number P12526
GeneID 25153
Peptideconfirmation Confirmedbyaminoacidanalysisandmassspectrometry.
Homology Mouse,human–identical.
RRID AB_2756621.
Purity Affinitypurifiedonimmobilizedantigen.
Form Lyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3 .
Isotype GuineapigtotalIgG.
Storagebeforereconstitution Theantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
Reconstitution 25µl,50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
Antibodyconcentrationafterreconstitution 0.8mg/ml.
Storageafterreconstitution Thereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
Negativecontrolantigenstoragebeforereconstitution Lyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
Negativecontrolantigenreconstitution 100µldoubledistilledwater(DDW).
Negativecontrolantigenstorageafterreconstitution -20°C.
PreadsorptionControl 1µgpeptideper1µgantibody.
Standardqualitycontrolofeachlot Westernblotanalysis.
Applications: wb
Mayalsoworkin: ic* ,ifc* ,ih* ,ip*
Westernblot- + Westernblotanalysisofratbrainlysate(lanes1and3)andmousebrainmembranes(lanes2and4):
1,2.Guineapig Anti-Angiotensin-(1-7)MasReceptor Antibody (#AGP-099),(1:600).
References
1.Santos,R.A.etal. (2003)Proc.Natl.Acad.Sci.U.S.A. 100, 8258. 2.Alenina,N.etal. (2008)Exp.Physiol. 93, 528. 3.Santos,R.A.etal. (2008)Exp.Physiol. 93, 519. Scientificbackground- + TheAngiotensin-(1-7)MasreceptoristherecentlyidentifiedreceptoroftheBIOLOG 1 2,3 q/11 proteinthatactivatesphospholipaseC(PLC).2,3 2
LyophilizedPowder NegativeControlAntigenIncluded
Clickhereto receivea25µl freetrialsample!
Lastupdate:14/08/2019
Alomone GuineapigAnti-Angiotensin-(1-7)MasReceptorAntibody (#AGP-099)raisedinguineapigcanbeusedinwesternblotanalysis.IthasbeendesignedtorecognizeAng-(1-7)Masreceptorfromrat,mouseandhumansamples.TheantigenusedtoimmunizeguineapigsisthesameasAnti-Angiotensin-(1-7)MasReceptorAntibody(#AAR-013)raisedinrabbit.Ourlineofguineapigantibodiesenablesmoreflexibilitywithourproductssuchasimmuno-colocalizationstudies,immunoprecipitation,etc.
Forresearchpurposesonly,notforhumanuse
Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。
LyophilizedPowder
AntigenIncl.
Westernblotanalysisofratbrainlysate(lanes1and3)andmousebrainmembranes(lanes2and4):1,2.Guineapig Anti-Angiotensin-(1-7)MasReceptor Antibody(#AGP-099),(1:600).
