BioX cell/InVivoMAb antimouse CD3ε F(ab')2 fragment/BE00011FAB/25MG相关交流-蚂蚁淘在线

BioX cell/InVivoMAb antimouse CD3ε F(ab')2 fragment/BE00011FAB/25MG

mayitao /发布时间:1528530925 /浏览量:104

Theanti-mouseCD3F(ab)2fragmentistheantigenbindingF(ab)2fragmentsofthe145-2C11monoclonalantibodyonly.ThemajorityoftheFcfragmenthasbeenremovedviapepsindigestion.The145-2C11antibodyF(ab)2fragmentreactswithmouseCD3ε,a20kDatransmembranecell-surfaceproteinthatbelongstotheimmunoglobulinsuperfamily.CD3εisoneoffivepolypeptidechainsthatcombinetoformtheTCRcomplex.CD3εisexpressedonTlymphocytes,NK-Tcells,andtovaryingdegreesondevelopingthymocytes.CD3playsrolesinTCRsignaling,Tlymphocyteactivation,andantigenrecognition.Thecomplete145-2C11antibodyiscommonlyusedtostimulateTcellsinvitrohowever,whenusedinvivoactivationofrestingTcellsresultsincytokinereleaseandtoxicitycausedbyAb-mediatedcross-linkingofTcellsandFcγreceptor-bearingcells.Toavoidthesecomplicationsnon-FcreceptorbindingF(ab’)2fragmentsofthe145-2C11antibodyarecommonlyused.Thisnon-FcR-bindinganti-CD3inducesapoptosisofAg-activatedTcellsinvivo byallowingdurableexpressionoftheTCRandsustainedsignaling.Foxp3⁺TregshavebeenshowntoberesistanttoCD3antibody-mediateddepletion.

Isotype	ArmenianHamsterIgG1
RecomendedIsotypeControl(s)	InVivoMAbhamsterIgGf(ab)2fragments
RecommendedInVivoPureDilutionBuffer	InVivoPurepH7.0DilutionBuffer
Immunogen	MouseBM10-37cytotoxicTcells
ReportedApplications	invivoTcelldepletion
Endotoxin	<2EU/mg(<0.002EU/μg) DeterminedbyLALgelclottingassay
Purity	>95% DeterminedbySDS-PAGE
Formulation	PBS,pH7.0 Containsnostabilizersorpreservatives
Sterility	0.2μMfiltered
Production	PepsinDigest
Purification	ProteinA
Storage	Undilutedat4°Cinthedark

References

SDSSheet

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