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BioBasic/Linear Poly Acrylamide (LPA) Solution (5mg/ml)/1ml/LK548

价格
¥370.80
货号:LK548
浏览量:41
品牌:BioBasic
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商品描述
Product Description:Linear Poly Acrylamide Solution (5mg/ml): The purification of nucleic acids for PCR, RT-PCR and other enzymatic reactions by alcohol precipitation is an essential step to yield high-quality results. Traditional alcohol precipitation procedures require co-precipitants like glycogen or yeast RNA to improve the recovery of nucleic acids. Recently, linear polyacrylamide has been shown to effectively precipitate nucleic acids (down to picogram quantities) when used with ethanol. Specifically, DNA fragments >20 base pairs have been shown to precipitate from solution while shorter fragments and free nucleotides remain in the supernatant.The addition of linear acrylamide (a neutral carrier) during alcohol precipitation offers several advantages to the more popular precipitation procedures using glycogen or yeast RNA. Because glycogen, yeast RNA and other popular co-precipitants are either derived from biological sources or treated with reagents derived from biological sources, there is the potential of trace nucleic acid contamination or nuclease contamination from the source.The introduction of minor contamination may lead to amplification of the background or worse, degradation of the final precipitation product. Linear acrylamide eliminates the risk of trace contaminants being introduced to the precipitation process because it is chemically synthesized and rigorously tested for nuclease contamination. Linear acrylamide also will not interfere with spectrophotometric readings at 260nm and 280nm. These clear advantages make linear acrylamide the best choice for precipitating DNA and RNA for amplification reactions.
Total Product Size:1ml
Individual Container Size:1ml
Number of Containers:1
Refrigeration Requirements:Refrigerator
Shipping Conditions:ICE
UNSPSC Code:41105500
UNSPSC Category:Nucleic Acid Purification Reagents
Bio Basic(又称“ Bio Basic Group”和“ Bio Basic Canada”)于2004年在加拿大多伦多成立,最初是从其制造的生化试剂的分销开始的。继成功之后,Bio Basic从2008年开始迅速扩展其制造设施,包括寡核苷酸,基因和肽合成,DNA测序以及实验室塑料制品和分子生物学试剂盒等系列。从2009年起,Bio Basic通过引入蛋白质纯化和抗体合成来进一步扩展其服务范围,以完善其现有产品和服务。