Details
NarISource
Nocardiaargentinensis
ReagentsSupplied
CMXBuffer1
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1µgofAdenovirus2DNAin1hourinatotalreactionvolumeof50µl
StarActivity
No
IncubationTemperature
37°C
HeatInactivation
10minutesat65°C
AssayUnitSubstrate
Adenovirus2DNA
ReactionBuffer
CMXBuffer1:
10mMTris-HCl(pH7.5at37°C)
10mMMgCl2
1mMdithiothreitol
100µg/mlbovineserumalbumin
ActivityinBuffers
Buffer1: 100%
Buffer2: 50%
Buffer3:25%
Buffer4:100%
Buffer5:50%
StorageBuffer
10mMTris‑HCl(pH7.8at4°C)
0.1mMEDTA
50mMKCl
1mMdithiothreitol
500µg/mlbovineserumalbumin
0.01%TritonX-100™
50%(v/v)glycerol
AssayConditions
10mMTris‑HCl(pH7.5at37°C)
10mMMgCl2
1mMdithiothreitol
100 µg/mlbovineserumalbumin
1µgAdenovirus2DNA
Incubationisat37°Cfor1hourinatotalreactionvolumeof50µl
StorageConditions
Storeat-20°C
Shippedondryice
Downloads
CertificateofAnalysis PDF -CurrentLot
MSDSPDF -CurrentLot
Categories
RestrictionEndonucleases