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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Details
Source
Moraxellabovis
ReagentsSupplied
CMXBuffer1
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1µgofunmethylatedlamBDaDNAin1hourinatotalreactionvolumeof50 µl
StarActivity
Yes
IncubationTemperature
37°C
HeatInactivation
10minutesat65°C
AssayUnitSubstrate
unmethylatedlambdaDNA
ReactionBuffer
CMXBuffer1:
10mMTris-HCl(pH7.0at37°C)
10mMMgCl2
1mMDTT
Note:100ug/mlbovineserumalbuminisincludedintheassaybutaddedseparatelyfromthereactionbuffer
ActivityinBuffers
Buffer1: 100%
Buffer2:50%
Buffer3:25%
Buffer4:75%
Buffer5:75%
*Staractivity
StorageBuffer
10mMTris-HCl(pH7.5at4°C)
0.1mMEDTA
50mMKCl
1mMdithiothreitol
0.01%TritonX-100™
100ug/mlbovineserumalbumin
50%glycerol
AssayConditions
50mMTris‑HCl(pH7.0at37°C)
10mMMgCl2
100mMNaCl
1mMDTT
100 µg/mlBSA
1µgunmethylatedlambdaDNA
Incubationisat37°Cfor1hourinatotalreactionvolumeof50µl
StorageConditions
Storeat-20°C
Shippedondryice
Downloads
CertificateofAnalysisPDF -CurrentLot
MSDSPDF -CurrentLot
Categories
RestrictionEndonucleases
