Details
Source
HaemophilusinfluenzaeRd
ReagentsSupplied
CMXBuffer2
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1µgoflamBDaDNAin1hourinatotalreactionvolumeof50µl
StarActivity
Yes
IncubationTemperature
37°C
HeatInactivation
65°Cfor10minutes
AssayUnitSubstrate
LambdaDNA
ReactionBuffer
CMXBuffer2:
10mMTris-HCl(pH7.5at37°C)
10mMMgCl2
50mMNaCl
1mMdithiothreitol
100µg/mlbovineserumalbumin
ActivityinBuffers
Buffer1:<25%
Buffer2:100%
Buffer3:<25%
Buffer4: 50%
Buffer5:<25%
*Staractivity
StorageBuffer
10mMTris-HCl(pH7.4at4°C)
200mMNaCl
0.5mMEDTA
1mMDTT
0.5mg/mlbovineserumalbumin
5µg/mlPoly-L-lysine
0.1%TritonX-100™
50%(w/v)glycerol
AssayConditions
50mMTris‑HCl(pH7.5at37°C)
10mMMgCl2
50mMNaCl
1mMdithiothreitol
1µg/mlbovineserumalbumin
1µglambdaDNA
Reactionvolumeof50µl
StorageConditions
Storeat-20°C
Shippedondryice
Downloads
CertificateofAnalysisPDF -CurrentLot
MSDSPDF -CurrentLot