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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Details
Source
RecombinantE.colistrain
ReagentsSupplied
10XT4PolynucleotideKinaseReactionBuffer
Applications
- Catalyzesthetransferofthegamma-phosphateofATPtothe5"-OHterminusinDNAorRNA
- Contains3"-phosphataseactivity(1)
- Usedfor5"-endlabelingofnucleicacidspriortoDNAorRNAsequencing(2,3)
- PhosphorylatessyntheticlinkersandfragmentsofDNAorRNApriortoligation
- Labels5"-terminipriortopartialrestrictionenzymedigestion
UnitDefinition
Oneunitistheamountofenzymerequiredtotransfer1nmolofγ-phosphatefromATPtothe5"OHterminusofsalmonspermDNAat37°C in30minutes
HeatInactivation
15minutesat65ºC
Concentration
20-30Units/µl
StorageBuffer
50mMTrisHCl(pH7.6)
0.1mMEDTA
25mMKCl
5mMdithiothreitol
0.1µMATP
50%(v/v)glycerol
AssayConditions
70mMTris-HCl(pH7.6)
10mMMgCl2
5mMdithiothreitol
50ug5"-OHterminatedDNA(micrococcalnucleasetreatedsalmonspermDNA)
0.07mM[γ-32P]ATP
Reactionvolumeof100µl
StorageConditions
Storeat-20°C
Productshippedondryice
Downloads
CertificateofAnalysisPDF -CurrentLot
MSDSPDF -CurrentLot
References
(1)Richardson,C.C.(1971)ProgressinNucleicAcidsRes.andMol.Biol.2,815-828
(2)Maxam,A.andGilbert,W.(1977)Proc.Natl.Acad.Sci.USA74,560-56
(3)Donis-Keller,H.(1980)NucleicAcidsRes.8,3133-3142
