品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Description:C1q,Mouse,mAbJL-1
ThemonoclonalantibodyJL-1recognizesthecollagen-likeregion(CLR)ofmouseC1q,a459kDamoleculeconsistingofthreeindividualpolypeptidechains.TheantibodyhasbeengeneratedbyimmunizationofC1q-/-C57BL/6micewithpurifiedmouseC1q.
C1qformstogetherwithC1randC1stheC1macromolecule,thefirstcomponentoftheclassicalcomplementpathway.InteractionofimmunecomplexeswithC1qinducesaconformationalchangewithintheC1complex,whichresultsinactivationoftheclassicalpathway.C1qfunctionsasrecognitionunitbybindingtotheheavychainofIgGorIgM(FcgammaandFcmicro)providedthattheimmunoglobulinsareboundtotheirantigen.FurThermore,C1qcanalsorecognizemolecularpatternsassociatedwithpathogensanditcanbindtoapoptoticblebs,whereitactivatestheclassicalcomplementpathwayandmediatesphagocytosis.Assuch,C1qpromotestheclearanceofapoptoticcellsandsubsequentexposureofautoantigens,therebypreventingstimulationoftheimmunesystem.
C1qispredominantlyproducedbymacrophagesbutalsobyfolliculardendriticcells,interdigitatingcellsandcellsofthemonocyte-macrophagelineage.C1qdeficiencyhasaprofoundeffectonhostdefenceandclearanceofimmunecomplexes.AbsenceofC1qmaycauseautoimmunitybyimpairmentoftheclearanceofapoptoticcells.InheritedC1qdeficiencyisalsoassociatedwiththedevelopmentofsystemiclupuserythematosus(SLE).
ThemonoclonalantibodyJL-1isreactivewiththecollagen-likeregion(CLR)only,whichisthesameregiontowhichautoantibodiesinmiceandhumansarebinding.Anti-C1qautoantibodiesdepositinglomerulitogetherwithC1qbutinduceovertrenaldiseaseonlyinthecontextofglomerularimmunecomplexdisease.Thisprovidesanexplanationwhyanti-C1qantibodiesareespeciallypathogenicinpatientswithSLE.
C1qformstogetherwithC1randC1stheC1macromolecule,thefirstcomponentoftheclassicalcomplementpathway.InteractionofimmunecomplexeswithC1qinducesaconformationalchangewithintheC1complex,whichresultsinactivationoftheclassicalpathway.C1qfunctionsasrecognitionunitbybindingtotheheavychainofIgGorIgM(FcgammaandFcmicro)providedthattheimmunoglobulinsareboundtotheirantigen.FurThermore,C1qcanalsorecognizemolecularpatternsassociatedwithpathogensanditcanbindtoapoptoticblebs,whereitactivatestheclassicalcomplementpathwayandmediatesphagocytosis.Assuch,C1qpromotestheclearanceofapoptoticcellsandsubsequentexposureofautoantigens,therebypreventingstimulationoftheimmunesystem.
C1qispredominantlyproducedbymacrophagesbutalsobyfolliculardendriticcells,interdigitatingcellsandcellsofthemonocyte-macrophagelineage.C1qdeficiencyhasaprofoundeffectonhostdefenceandclearanceofimmunecomplexes.AbsenceofC1qmaycauseautoimmunitybyimpairmentoftheclearanceofapoptoticcells.InheritedC1qdeficiencyisalsoassociatedwiththedevelopmentofsystemiclupuserythematosus(SLE).
ThemonoclonalantibodyJL-1isreactivewiththecollagen-likeregion(CLR)only,whichisthesameregiontowhichautoantibodiesinmiceandhumansarebinding.Anti-C1qautoantibodiesdepositinglomerulitogetherwithC1qbutinduceovertrenaldiseaseonlyinthecontextofglomerularimmunecomplexdisease.Thisprovidesanexplanationwhyanti-C1qantibodiesareespeciallypathogenicinpatientswithSLE.
Specifications:
| Catalognumber | HM1096 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100μg/ml)0.2μmfilteredproteinGpurifiedantibodysolutioninPBS,containing0.1%bovineserumalbumin.Theendotoxinconcentrationis<24EU/mg,determinedwithHIT302LALAssay. |
| Application | Frozensections,Functionalstudies,Immunoassays,Immunofluorescence,Westernblot |
| ApplicationNotes | IHC-F:AntibodyJL-1wasusedtostaintissuesectionswhichwerefixedinacetone.Aspositivecontrolapolyclonalanti-C1qantibodywasusedandasnegativecontrolanisotypematchedmonoclonalantibody(Ref.1). FS:AntibodyJL-1wasadmiNISTeredtomiceresultingindepletionofcirculatingC1q,glomerulardepositionofC1qandinductionofanti-C1qautoantibodiesinsusceptIBLemice.Asanegativecontrolanisotypematchedmonoclonalantibodywasused(Ref.1). |
| Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Immunogen | PurifiedmouseC1q |
| Isotype | MouseIgG2b |
| Species | Mouse |
| Crossreactivity | Human-Yes,Rat-Yes |
| Alias | ComplementcomponentC1q,ComplementC1qsubcomponentsubunitA |
| PositiveControl | Spleenandkidneytissueofwild-typemice(Ref.1) |
| NegativeControl | SpleenandkidneytissueofC1q-/-mice(Ref.1) |
| References | 1.Trouw,Letal;Anti-C1qautoantibodiesdepositinglomerulibutareonlypathogenicincombinationwithglomerularC1q-containingimmunecomplexes.JClinInvest2004,114:679 2.Li,Metal;DevelopmentofahumanizedC1qAchainknock-inmouse:assessmentofantibodyindependentbeta-amyloidinducedcomplementactivation.MolImmunol2008,45:3244 3.Erlich,Petal;ComplementproteinC1qformsacomplexwithcytotoxicprionproteinoligomers.JBiolChem2010,285:19267 |
| StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsibleforanypatentinfringementsthatmightresultwiththeuseorderivationofthisproduct. |
| Disease | Nephrology,Neurologicaldisorders |
