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苏州工业园区生物纳米园A4#216
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商品描述
Description:Dectin-1,Mouse,mAb2A11
Themonoclonalantibody2A11recognizesmouseβ-glucanreceptor,alsoknownasdectin-1.Dectin-1isasmalltypeIItransmembranereceptorcontainingonelectin-likecarbohydraterecognitiondomain.Thisdomainrecognizesβ1,3-and/orβ1,6-linkedglucans,intactyeastandanimmunoreceptortyrosine-basedactivationmotif(ITAM)inthecytoplasmictail.β-Glucanisoneofthemostabundantpolysaccharidesinfungalpathogens.Byrecognizingβ-glucan,dectin-1hasafundamentalfunctioninantifungalimmunity.Dectin-1isasignalingnon–Toll-likepattern-recognitionreceptorrequiredfortheinductionofprotectiveimmuneresponses.Dectin-1isresponsIBLefortheβ-glucan-dependent,nonopsonicrecognitionofzymosanbyprimarymacrophages.Zymosanisayeastderivedparticlecomposedprincipallyofpolysaccharides,ofwhichβ-glucanandmannanarethemajorconstituents.InvivoadmiNISTrationofzymosan,orpurifiedsolubleβ-glucans,hasanumberofdesirableeffectsonimmunefunction,includingtheABIlitytoconferresistancetotumorsandvariousinfections.ThereceptorishighlyexpressedonmacrophagesandneutrophilsandtoalesserextentondendriticcellsandasubpopulationofTcells.Dectin-1participateswiththecomplementreceptorCR3inahostproinflammatorycytokineresponsetoafungalpathogen.Themonoclonalantibody2A11recognizesanextracellularepitopeondectin-1.
Specifications:
Catalognumber | HM1067 |
---|---|
Producttype | Monoclonalantibodies |
Quantity | 100µg |
Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbumin. |
Application | Flowcytometry,Frozensections,Functionalstudies,Immunoprecipitation |
ApplicationNotes | FC:Antibody2A11stainstheextracellulardomainofmousedectin-1.ForintracellularstainingmousemacrophageswerepermeabilizedwithIntraPrep.Themacrophageswereincubatedwith1 µg/mlantibodyfor1hrat4 °C.(Ref.4) IHC-F:-TissuesectionswerefrozeninTissue-Tekandfixedinacetone.Aspositivecontrollymphnodeswereused(Ref.5). FS:Antibody2A11functionsasinhibitorofphagocytosisbyzymosan.Theantibodywasfunctionallytestedbyendocytosisassays.TheBIOLOGicalactivityoftheantibodycanbedefinedasthepercentagephagocytosisinhibition(Ref.7). |
Use | Forimmunohistochemistryandflowcytometry,dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.Forfunctionalstudies,invitrodilutionshavetobeoptimizedinuser’sexperimentalsetting. |
Immunogen | NIH3T3cellstransducedwithfull-lengthDectin-1 |
Isotype | RatIgG2b |
Species | Mouse |
Alias | beta-glucanreceptor |
PositiveControl | Mouseprimarymacrophages |
References | 1.Brown,Getal;Dectin-1isamajorβ-glucanreceptoronmacrophages.JExpMed2002,196:407 2.Taylor,Petal;Theβ-glucanreceptor,dectin-1,ispredominantlyexpressedonthesurfaceofcellsofthemonocyte/macrophageandneutrophillineages.JImmunol2002,169:3876 3.Brown,Getal;Dectin-1mediatesthebiologicaleffectsofβ-glucans.JExpMed2003,197:1119 4.Diniz,Setal;PTX3functionasanopsoninforthedectin-1-dependentinternalizationofzymosanbymacrophages.JLeukocBiol2004,75:649 5.Dupasquier,Metal;ThedermalmicroenvironmentinducestheexpressionofthealternativeactivationMarkerCD301/mMGLinmononuclearphagocytes,independentofIL-4/IL-13signaling.JLeukocBiol2006,80:838 6.Huysamen,Cetal;CLEC9AisanovelactivationC-typelectin-likereceptorexpressedonBDCA3+dendriticcellsandasubsetofmonocytes.JBiolChem2008,383:16693 7.Lin,J-Setal;Distinctrolesofcomplementreceptor3,Dectin-1,andsialicacidsinmurinemacrophageinteractionwithHistoplasmayeast.JLeukocBiol2010,88:1 |
Storageandstability | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforatleastoneyear.Theexactexpirydateisindicatedonthelabel. |
Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsibleforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
Disease | Infectiousdiseases,Tumorimmunology |