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商品描述
Description:SR-A,Mouse,mAb2F8
Themonoclonalantibody2F8recognizesmousescavengerreceptorclassA(SR-A),typeIandII,alsoknownasCD204.SR-Abelongstothegroupofscavengerreceptors,whicharedividedinclassA,B,DandE.ClassAscavengerreceptors(SRs)aremulti-domainedmembraneglycoproteinsthatcanendocytosemodifiedlipoproteinsandbindawiderangeofligands.SR-Aisexpressedbytissuemacrophagesandfunctionsbothasanendocyticreceptorforlipoproteinsandasanadhesionreceptorformacrophagesbindingtoligandrichtissuese.g.atheroscleroticlesions.Tissuemacrophage-populationsthatexpressSR-AIandIIincludethoseofthemarginalzoneofthespleen,alveolarmacrophages,heartandgut.
SR-Abindsbacterialpathogensandisconsideredtoplayaroleintheinnateimmuneresponse.TLR2andTLR4agoNISTssynergisticallyup-regulateSR-AinRAW264.7throughp38pathway.SR-AknockoutmicearemoresusceptIBLetoendotoxicshockthanwildtypemice,whichsuggestsaroleintheclearanceofbacterialendotoxinfromthecirculation.UsingthymicmacrophagesoftheknockoutmiceaswellasantibodytreatmentconfirmedthatSR-Aisresponsibleforhalfofthephagocyticactivityrequiredforapoptoticthymocyteingestion.NexttoitsroleininnateimmunitythereisevidencethatSR-Aalsocontributestoacquiredimmuneresponses.
Monoclonalantibody2F8recognizesanepitopewithintheαhelicalcoiled-coildomainofSR-Ainallmousestraintested,withtheexceptionofC57BL/6.Oneamino-acidchange(L168S),duetopolymorphism,resultsinthelossofreactivityofantibody2F8.
SR-Abindsbacterialpathogensandisconsideredtoplayaroleintheinnateimmuneresponse.TLR2andTLR4agoNISTssynergisticallyup-regulateSR-AinRAW264.7throughp38pathway.SR-AknockoutmicearemoresusceptIBLetoendotoxicshockthanwildtypemice,whichsuggestsaroleintheclearanceofbacterialendotoxinfromthecirculation.UsingthymicmacrophagesoftheknockoutmiceaswellasantibodytreatmentconfirmedthatSR-Aisresponsibleforhalfofthephagocyticactivityrequiredforapoptoticthymocyteingestion.NexttoitsroleininnateimmunitythereisevidencethatSR-Aalsocontributestoacquiredimmuneresponses.
Monoclonalantibody2F8recognizesanepitopewithintheαhelicalcoiled-coildomainofSR-Ainallmousestraintested,withtheexceptionofC57BL/6.Oneamino-acidchange(L168S),duetopolymorphism,resultsinthelossofreactivityofantibody2F8.
Specifications:
Catalognumber | HM1061 |
---|---|
Producttype | Monoclonalantibodies |
Quantity | 100µg |
Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbumin. |
Application | Flowcytometry,Frozensections,Functionalstudies,Immunoprecipitation,Westernblot |
ApplicationNotes | FC:Antibody2F8stainstheextracellulardomainofmouseSR-A.RAW.264cellswerefixedinparaformaldehydebeforestaining.AspositivecontrolRAW.264cellswereusedandasnegativecontrolisotypematchedantibody. W:Anon-reducedsampletreatmentandSDS-PAGEwasused.Thebandsizesare156-238kDa(Ref.5). IHC-F:Tissuesectionswerefixedin2%paraformaldehydeinHepesbufferedsalineandpretreatedwith0.01Mglucose,0.001MNaN3,40UglucoseoxidaseinPBSfor15minutesat37 °Ctoquenchendogenousperoxidases.Aspositivecontrolspleenwasusedandasnegativecontrolisotypematchedantibody(Ref.3). FS:Antibody2F8-functionsasinhibitorofEDTA-resistantadhesionofmacrophagestofrozentissuesection.FurThermore,itantagonizesacetylatedlow-densitylipoprotein-inducedcholesterolesterificationinMPM.TheBIOLOGicalactivityoftheantibodycanbedefinedastheABIlitytoinhibitthedivalentcation-independentcomponentofmacrophageadhesion(Ref.3,5). |
Use | Forimmunohistochemistry,flowcytometryandWesternblottingdilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.Forfunctionalstudies,invitrodilutionshavetobeoptimizedinuser’sexperimentalsetting. |
Immunogen | RAW.264cells(αhelicalcoiledcoildomainCD204) |
Isotype | RatIgG2b |
Species | Mouse |
Alias | CD204 |
PositiveControl | RAW.264cells |
References | 1.Fraser,Ietal;Divalentcation-independentmacrophageadhesioninhibitedbymonoclonalantibodytomurinescavengerreceptor.Nature1993,364:343 2.Bell,Detal;UpregulationofthemacrophagescavengerreceptorinresponsetodifferentformsofinjuryintheCNS.JNeurocytol1994,10:605 3.Hughes,Detal;MurineMscavengerreceptor:adhesionfunctionandexpression.ImmunolLett1994,43:7 4.Hughes,Detal;Murinemacrophagescavengerreceptor:invivoexpressionandfunctionasreceptorformacrophageadhesioninlymphoidandnon-lymphoidorgans.EurJImmunol1995,25:466 5.Daugherty,Aetal;PolymorphismofclassAscavengerreceptorsinC57BL/6mice.JLipidRes2000,41:1568 6.Knapp,Setal;Pulmonarylipopolysaccharide(LPS)-bindingproteininhibitstheLPS-inducedlunginflammationinvivo.JImmunol2006,176:3189 |
Storageandstability | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforatleastoneyear.Theexactexpirydateisindicatedonthelabel. |
Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsibleforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
Disease | Infectiousdiseases |