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商品描述
Description:CD14,Mouse,mAbSa14-2,FITC
ThemonoclonalantibodySa14-2recognizesthemousemonocyteMarkerCD14.TheCD14receptorisapatternrecognitionmoleculeintheinnateimmuneresponseagainstmicroorganismsandotherexogenousandendogenousstressfactors.CD14wascharacterizedasareceptorforLPS.TheCD14geneconsistsoftwoexonswhichcodeforasinglemRNAthatistranslatedintoaproteinof375aminoacids.TheCD14proteiniscomposedofelevenleucin-richrepeats,whicharealsofoundinTLRandwhichareimportantinPAMPbinding.IncontrasttoTLR,however,CD14lacksatransmembranedomain,andthuscannotinitiateintracellularsignaltransductionbyitself.ThemostimportantCD14signalingco-receptoristoll-likereceptor4(TLR4),whichactivates,amongothers,thenuclearfactorκB(NF-κB)inflammatorypathway.TheCD14proteinisprocessedintheendoplasmaticreticulumandexpressedasa55kDaglycoproteinonthecellsurfaceviaaglycosylphosphatidyl(GPI)anchor.LikeotherGPI-anchoredproteins,CD14accumulatesonthecellsurfaceinmicrodomainsknownaslipidrafts.CD14isexpressedpredominantlyonthesurfaceof"myeloid"cells,suchasmonocytes,macrophagesandneutrophils,butatlowerlevelsalsoonepithelialcells,endothelialcellsandfibroblasts.
CD14isalsoexpressedinasolubleform(sCD14).sCD14mayresultfromsecretionoftheproteinbeforecouplingtotheGPIanchororfromsheddingorcleavagefromthesurfaceofmonocytes.sCD14ispresentinthecirculationandotherbodyfluidsandlevelsofsCD14inplasmaincreaseduringinflammationandinfection.CD14isamoleculewithabroadrangeoffunctions.Inadditiontofunctioningasapatternrecognitionreceptorforavarietyofmicrobialligands,CD14alsoactsasareceptorforendogenousmoleculeslikeintercellularadhesionmolecule(ICAM)-3onthesurfaceofapoptoticcells,amyloidpeptid,ceramide,anduratecrystals.LigationofCD14bytheseligands,exceptforapoptoticcells,mediatesactivationofinflammatoryresponses.
CD14isalsoexpressedinasolubleform(sCD14).sCD14mayresultfromsecretionoftheproteinbeforecouplingtotheGPIanchororfromsheddingorcleavagefromthesurfaceofmonocytes.sCD14ispresentinthecirculationandotherbodyfluidsandlevelsofsCD14inplasmaincreaseduringinflammationandinfection.CD14isamoleculewithabroadrangeoffunctions.Inadditiontofunctioningasapatternrecognitionreceptorforavarietyofmicrobialligands,CD14alsoactsasareceptorforendogenousmoleculeslikeintercellularadhesionmolecule(ICAM)-3onthesurfaceofapoptoticcells,amyloidpeptid,ceramide,anduratecrystals.LigationofCD14bytheseligands,exceptforapoptoticcells,mediatesactivationofinflammatoryresponses.
Specifications:
Catalognumber | HM1060F |
---|---|
Producttype | Monoclonalantibodies |
Quantity | 100µg |
Formulation | 1ml(100μg/ml)0.2μmproteinGpurifiedFITCconjugatedantibodysolutioninPBS,containing1%bovineserumalbuminand0.02%sodiumazide. |
Conjugate | FITC |
Application | Flowcytometry,Functionalstudies,Immunofluorescence |
Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
Immunogen | CD14transfectant |
Isotype | RatIgG2a |
Species | Mouse |
Alias | MonocytedifferentiationantigenCD14,Myeloidcell-specificleucine-richglycoprotein,CD14 Genename:Cd14 |
PositiveControl | RAW-cells |
References | 1.Akashi,Setal;LipopolysaccharideinteractionwithcellsurfaceToll-likereceptor4-MD-2:higheraffinitythanthatwithMD-2orCD14.I.Exp.Med.2003,198:1035-1042 2.Hajishengallis,Getal;Porphyromonasgingivalisfimbriaeproactivelymodulatebeta2integrinadhesiveactivityandpromotebindingtoandinternalizationbymacrophages.InfectImmun2006,74:5658 3.Kiyokawa,Tetal.AsinglebasemutationinthePRAT4AgenerevealsdifferentialinteractionofPRAT4AwithToll-likereceptors.IntImmunol2008,20:1407-1415 4.Wilkinson,Tetal;Trappin-2promotesearlyclearanceofpseudomonasaeruginosathroughCD14-dependentmacrophageactivationandneutrophilrecruitment.AmJPathol2009,174:1338 |
StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforatleastoneyear. |
Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
Disease | Infectiousdiseases |