PuromycindihydrochlorideisanaminonucleosideantibioticderivedfromStreptomycesalboniger.Puromycindihydrochloride isroutinelyusedasaselectiveagentintransfectionandtransformationprotocols.

Formorepuromycinproducts,clickhere.

Contietal. usedpuromycinDiHClfromTOKU-EtoselectforeGFPexpressingA549cells. "PolymericNanocarriersAndTheirOralInhalationForumlationsForTheRegionalDeliveryofNucleicAcidsToTheLungs."

Sandoval-Jaimeetal. usedpuromycinDiHClfromTOKU-Etoselectforstablytransfectedcells. "RecoveryofmurinenorovirusandfelinecalicivirusfromplasmidsencodingEMCVIRESinstablecelllinesexpressingT7polymerase."

Mutongaetal.usedpuromycinfromTOKU-EtoselectforresistantcellstransformedwithavectorcontainingSUV39H2(ahistonemethyltransferase)andapuromycinresistancegene."TargetingSuppressorofVariegation3-9Homologue2(SUV39H2)inAcuteLymphoblasticLeukemia(ALL)."

Luetal. usedblasticidinSHClandpuromycindihydrochloridefromTOKU-EtoselectfortransfectedAS-B145andBT-474cells. "OvatodiolideInhibitsBreastCancerStem/ProgenitorCellsthroughSMURF2-MediatedDownregulationofHsp27" 

CASNumber

58-58-2

MolecularFormula

C₂₂H₂₉N₇O₅·2HCl

MolecularWeight

544.43

MechanismofAction

Duringtranslation,puromycinenterstheribosomal“A”siteanddisruptspeptidetransfer.Asaresult,theribosomestopsandthepeptidechainisterminatedleADIngtoanonfunctionalprotein.

Mechanismofresistance

PuromycinresistanceisconferredbythepacgeneandisisolatedfromStreptomycesalboniger.Thepacgeneisa600nucleotidefragmentofDNAandencodespuromycinN-acetyltransferase.PuromycinN-acetyltransferaseinactivatespuromycinbyacetylatingtheaminogroupinthepuromycintyrosinylmoiety.AcetylatedpuromycinisBIOLOGicallyinactiveanddoesnotassociatewithprokaryoticoreurkaryoticribosomes.

StorageConditions

2-8°C

TariffCode

2941.90.1010

Spectrum

Puromycindihydrochlorideisactiveagainstbothprokaryoticandeurkaryoticcells.

Specifications:

Form

Powder

Appearance

Whiteoroff-whitepowder

WaterContent(KarlFisher)

≤12.0%

MeltingPoint

168-178°C

TOKU-E植物生物学植物生物学，基因工程，改变植物生长特性。植物生物学是指植物的科学和研究。植物的特性和生长可以通过多种方式进行改变，包括基因工程和各种生长调节剂或激素的使用。可以通过农杆菌介导的转化或其他方法将DNA导入植物，  其中将质粒上的目标基因从农杆菌  细胞中转移  并整合到植物组织中。植物还易受细菌和真菌性植物病原体的侵害，而使用低毒性抗生素  和  抗真菌药可以控制或消除这些病原体  。