ProductHighlights:
WiththeInfiniumMethylationEPICBeadChipKit,researcherscaninterrogateover850,000methylationsitesquantitativelyacrossthegenomeatsingle-nucleotideresolution.Multiplesamples,includingFFPE,canbeanalyzedinparalleltodeliverhigh-throughputpowerwhileminimizingthecostpersample.
InfiniumHDarraytechnologyenablescontentselectionindependentofbias-associatedlimitationsoftenassociatedwithmethylatedDNAcapturemethods.Theresultisapan-enhancerandcodingregionviewofthemethylomethatcanbeusedforepigenome-wideassociationstudiesonavarietyofhumantissues.
ComprehensiveGenome-WideCoverage
Thiskitincludesthefollowingcontentcategoriesrequestedbymethylationexperts:
- CpGsitesoutsideofCpGislands
- Non-CpGmethylatedsitesidentifiedinhumanstemcells(CHHsites)
- Differentiallymethylatedsitesidentifiedintumorversusnormal
- FANTOM5enhancers
- ENCODEopenchromatinandenhancers
- DNasehypersensitivesites
- miRNApromoterregions
- >90%ofcontentcontainedontheIlluminaHumanMethylation450KBeadChip
IntegratedAnalysisSoftware
MethylationEPICdataanalysisissupportedbytheGenomeStudioMethylationModule.GenomeStudiosoftwareenablesresearcherstoeffortlesslyperformdifferentialmethylationanalysisforsmall-scalestudies.Itfeaturesadvancedvisualizationtoolsthatenableresearcherstoviewvastamountsofdatainasinglegraph,suchasheatmaps,scatterplots,andlineplots.
ThesetoolsandtheGenomeStudioGenomeBrowserdisplayvaluableinformationsuchaschromosomalcoordinates,percentGC,locationinaCpGIsland,andmethylationβvalues.
Forlarge-scalestudies,therearemanyfreeanalysispackagesthatworkinthesoftwareframeworkRfornormalization,anddifferentialanalysisofmethylationdata.Instructionalvideosexplaintheuseofthesedataanalysispackages.
Specifications:
NumberofMarkers | Over850,000methylationsitespersampleatsingle-nucleotideresolution |
InputQuantity | 250ngDNA |
Technology | Microarray |
SpecializedSampleTypes | FFPE,LowInput |
VariantClass | DifferentiallyMethylatedCytosines |
SpeciesCategory | Human |
SystemCompatibility | iScan |