ProductHighlights:
TheTruSeqSyntheticLong-ReadDNALibraryPrepKitisahighlyaccurate,end-to-endsequencingsolutionthatcanbeusedforgenomeassemblyorgenomephasing.Dependingontheanalysisoptionyouselect,youcanuseitto:
- Assemblesyntheticallylongreadsfordenovoassemblyandgenomefinishingapplications
- Performgenomephasingtoidentifyco-inheritedallelesandhaplotypeinformation,aswellasphasedenovomutations
AccuratelyConstructSyntheticLongReads
TheTruSeqSyntheticLong-ReadDNALibraryPrepandTruSeqSyntheticLong-ReadDNABarcodeKitsaredesignedforpreparingDNAlibrariestogeneratesyntheticallylongreads.ThelibraryprepkitcombinesTruSeqandNexterachemistrieswithsyntheticlong-readtechnologytoprepareDNAlibraries.Theaccompanyingbarcodekitincludes384indexesforlabelingthesamplesineachwell.Theseindexesarethenusedaftersequencingtoconstructsyntheticallylongfragmentsforlong-readassemblyandphasinganalysis.
SimplifiedInformaticsforGenomeAssemblyorHumanWhole-GenomePhasing
Aftersequencing,push-buttonanalysisinBaseSpaceSequenceHubsimplifiesassemblyoflongreads.DatacanbetransferredfromanIlluminasequencinginstrumenttotheBaseSpaceSequenceHubcloudinstantly.
BaseSpaceAppforGenomeAssembly:
TheTruSeqLong-ReadAssemblyAppconstructslong,syntheticreadsfromshortersequencingreadsforaccurategenomeassemblyandgenomefinishing.TruSeqsyntheticlong-readtechnologyallowsyoutousethesamefamiliarplatformwithanewapplication.
BaseSpaceAppforPhasingAnalysis:
TheTruSeqPhasingAnalysisAppcanbeusedtoperformwholehumangenomephasing,identifyinghaplotypeinformationandco-inheritedalleles,andphasingdenovomutations.Byconstructingsyntheticallylongfragmentsfromshortersequencingreads,thismethodprovidesmorecomprehensiveandaccuratephasingcomparedtoconventionaltriostudiesorstatisticalinference.
Specifications:
AssayTime | 3days |
Hands-OnTime | 6hours |
SpeciesCategory | AnySpecies |
SystemCompatibility | HiSeq2000,HiSeq3000,HiSeq2500,HiSeq4000 |
Method | Whole-GenomeSequencing,DeNovoSequencing,Long-ReadSequencing |
Technology | Sequencing |