ProductHighlights:
TheTruSeqStrandedmRNALibraryPrepKitoffersastreamlined,cost-efficient,andscalablesolutionforcodingtranscriptomeanalysis.ItiscompatIBLewithawiderangeofsamples.
PreciseandAccurate
GetprecisemeasurementofmRNAstrandorientationfordetectionofantisensetranscription,enhancedtranscriptannotation,andincreasedalignmentefficiency.Highcoverageuniformityenhancesthediscoveryoffeaturessuchasalternativetranscripts,genefusions,andallele-specificexpression.
Cost-Efficient
Strandedinformationidentifiesfromwhichofthe2DNAstrandsagivenRNAtranscriptwasderived.Thisinformationprovidesincreasedconfidenceintranscriptannotation,particularlyfornonhumansamples.Identifyingstrandoriginincreasesthepercentageofreadsthatalign,reducingsequencingcostspersample.
Scalable
Thekitenablesrobustinterrogationofbothstandardandlow-qualitysamples,andworkflowscompatiblewithawiderangeofstudydesigns.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatiblewiththislibrarypreparationkit
Specifications:
AssayTime | ~10.5hours |
Hands-OnTime | ~4.5hours |
InputQuantity | 0.1–1ugtotalRNAor10-100ngpreviouslyisolatedmRNA(fromspecieswithpolyAtails) |
ContentSpecifications | Capturesthecodingtranscriptomewithstrandinformation |
MechanismofAction | Oligo-dTbeadscapturepolyAtails |
SystemCompatibility | NovaSeq5000,GenomeAnalyzerIIx,HiSeq2000,MiSeq,NextSeq550,HiSeq3000,HiSeq1000,HiSeq1500,NextSeq500,HiSeq2500,NovaSeq6000,HiSeq4000 |
SpeciesCategory | Other,Mammalian,Bovine,Mouse,Human,Rat |
VariantClass | SingleNucleotidePolymorphisms(SNPs),GeneFusions,NovelTranscripts,TranscriptVariants |
Technology | Sequencing |
Method | mRNASequencing |
AutomationCapABIlity | LiquidHandlingRobots |