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商品描述
HumanHistoneChaperoneASF1A(ASF1A)belongstotheH3/H4familyofhistonechaperoneproteins.ASF1Aisubiquitouslyexpressedinmanycellsandtissues,interactingwithhistonesH3andH4.ASF1AcooperateswithChromatinAssemblyFactor1topromotereplication-dependentchromatinassemblyandwithHIRAtopromotereplication-independentchromatinassembly.Inaddition,ASF1Aisnecessaryfortheformationofsenescence-associatedheterochromatinfoci(SAHF)andefficientsenescence-associatedcellcycleexit.
| Source | E.coli |
| Names | HistoneChaperoneASF1A,Anti-SilencingFunctionProtein1HomologA,hAsf1,hAsf1a,CCG1-InteractingFactorA,CIA,hCIA,ASF1A |
| Accession# | Q9Y294 |
| Formulation | Lyophilizedfroma0.2μMfilteredsolutionof20mMTris-HCl,1mMDTT,150mMNaCl,pH8.0 |
| Shipping | Theproductisshippedatambienttemperature. |
| Reconstitution | Alwayscentrifugetubesbeforeopening.Donotmixbyvortexorpipetting. Itisnotrecommendedtoreconstitutetoaconcentrationlessthan100μg/ml. Dissolvethelyophilizedproteinin1XPBS. Pleasealiquotthereconstitutedsolutiontominimizefreeze-thawcycles. |
| Storage | Lyophilizedproteinshouldbestoredat<-20°C,thoughstableatroomtemperaturefor3weeks. Reconstitutedproteinsolutioncanbestoredat4-7°Cfor2-7days. Aliquotsofreconstitutedsamplesarestableat<-20°Cfor3months. |
| Purity | Greaterthan95%asdeterminedbySEC-HPLCandreducingSDS-PAGE. |
| Endotoxin | Lessthan0.1ng/μg(1IEU/μg). |
| AminoAcidSequence | MASMTGGQQMGRGSMAKVQVNNVVVLDNPSPFYNPFQFEITFECIEDLSEDLEWKIIYVGSAESEEYDQVLDSVLVGPVPAGRHMFVFQADAPNPGLIPDADAVGVTVVLITCTYRGQEFIRVGYYVNNEYTETELRENPPVKPDFSKLQRNILASNPRVTRFHINWEDNTEKLEDAESSNPNLQSLLSTDALPSASKGWSTSENSLNVMLESHMDCMLEHHHHHH |
